肺微血管內皮細胞構成半選擇性屏障，該屏障對于肺氣體交換，調節液體和可溶物在血液與肺間質之間的流動具有重要意義。它還具有代謝功能，可以執行一定的非呼吸功能。在肺損傷中，PMECs是活性氧類的重要靶細胞之一。在肺炎的發生過程中，神經體液介質和氧化劑作用于內皮細胞，使得細胞間隙滲透性增加，蛋白質由血液進入間質。細胞間隙滲透性的增加導致低氧血癥，出現成人呼吸窘迫綜合征和非心源性肺水腫。
人肺微血管內皮細胞提取于人肺組織，細胞提取純化之后立即凍存。每管含有細胞數>5×105 cells/ml，此細胞通過vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗證，經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到10倍增。
推薦培養基：內皮細胞培養基 (ECM, Cat. #1001)
| 貨號 | 3000 |
| 產地 | 美國 |
| 縮寫 | HPMEC |
| 規格 | 5 x 10^5 /1ml |
| 用途 | 科研 |
| 儲存 | 液氮 |
| 運輸 | 干冰 |
1.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) 'Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains.'
2.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) "Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains."?Infect immune. 82: 949-59.
3.) Claessens A, Adams Y, Ghumra A, Lindergard G, Buchan CC, Andisi C, Bull PC, Mok S, Gupta AP, Wang CW, Turner L, Arman M, Raza A, Bozdech Z, Rowe JA. (2012) "A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells."?Proc Natl Acad Sci USA.109: E1772-81.?
4.) Kang CY, Zou L, Yuan M, Wang Y, Li TZ, Zhang Y, Wang JF, Li Y, Deng XW, Liu CT. (2011) "Impact of simulated microgravity on microvascular endothelial cell apoptosis."Eur J Appl Physiol. 111: 2131-8.
5.) Reutershan J, Saprito MS, Wu D, R?ckle T, Ley K. (2010) "Phosphoinositide 3-kinase gamma required for lipopolysaccharide-induced transepithelial neutrophil trafficking in the lung."?Eur Respir J. 35: 1137-47.?
6.) Rydkina E, Turpin LC, Sahni SK. (2010) "Rickettsia rickettsii infection of human macrovascular and microvascular endothelial cells reveals activation of both common and cell type-specific host response mechanisms."?Infect Immun. 78: 2599-606.
7.) Shahani T, Lavend'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. (2010) "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool."?Blood. 115: 4902-9.?
8.) Wright CJ, Agboke F, Chen F, LA P, Yang G, Dennery PA. (2010) "Nitric Oxide Inhibits Hyperoxia-induced NF-kappaB Activation in Neonatal Pulmonary Microvascular Endothelial Cells."?Pediatr Res. 68: 484-9.
9.) Oca?a-Macchi M, Bel M, Guzylack-Piriou L, Ruggli N, Liniger M, McCullough KC, Sakoda Y, Isoda N, Matrosovich M, Summerfield A. (2009) "Hemagglutinin-dependent tropism of H5N1 avian influenza virus for human endothelial cells."?J Virol. 83: 12947-55.?
10.) Wolters PJ, Wray C, Sutherland RE, Kim SS, Koff J, Mao Y, Frank JA. (2009) "Neutrophil-derived IL-6 limits alveolar barrier disruption in experimental ventilator-induced lung injury."?J Immunol. 182: 8056-62.?
11.) Stefanescu R, Bassett D, Modarresi R, Santiago F, Fakruddin M, Laurence J. (2008) "Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis."?Blood. 112: 340-9.
12.) Kiss J, Yegutkin GG, Koskinen K, Savunen T, Jalkanen S, Salmi M. (2007) "IFN-beta protects from vascular leakage via up-regulation of CD73."?Eur J Immunol. 37: 3334-8.
13.) Moldobaeva A, Welsh-Servinsky LE, Shimoda LA, Stephens RS, Verin AD, Tuder RM, Pearse DB. (2006) "Role of protein kinase G in barrier-protective effects of cGMP in human pulmonary artery endothelial cells."?Am J Physiol Lung Cell Mol Physiol. 290: L919-30.
14.) Kanmogne GD, Primeaux C, Grammas P. (2005) "Induction of apoptosis and endothelin-1 secretion in primary human lung endothelial cells by HIV-1 gp120 proteins."Biochem Biophys Res Commun. 333: 1107-15.
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