Puromycin is an aminonucleoside antibiotic isolated from Streptomyces alboniger. Puromycin terminates protein synthesis at the level of translation in prokaryotic and eukaryotic cells and is active against gram-positive bacteria and weakly active against gram-negative bacteria. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase (pac) gene [1, 2]. Exogenous expression of the pac gene is commonly used in conjunction with puromycin to generate stable cell lines. Most working concentrations range from 1-10 μg/ml; however, the optimal concentration should be determined for individual cell types and culture conditions. In most cases, puromycin kills 99% of non-resistant cells within 48 hours.
| 貨號 | 0553 |
| 產地 | 美國 |
| 縮寫 | PURO-1 |
| 規格 | 1 ml |
| 用途 | 科研 |
| 儲存 | -20度 |
| 運輸 | 干冰 |
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