99久久香蕉国产线看观看

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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        您當前所在位置:首 頁 > 產品展示 > 細胞檢測試劑盒 > 干細胞 茜素紅S染色量化分析試劑盒 ARed

        茜素紅S染色量化分析試劑盒 ARed

        英文名:Alizarin Red S Staining Quantification Assay

        貨號:8678

        價格:$170

        產品說明書下載:

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        Alizarin Red S (ARS), an anthraquinone dye, has been widely used to evaluate calcium deposits in cell culture. The ARS staining is quite versatile because the dye can be extracted from the stained monolayer of cells and readily assayed.

        ScienCell’s ARS Staining Quantification Assay (ARed-Q) provides a sensitive tool for the recovery and semi-quantification of ARS in a stained monolayer of cells. Mineralization is assessed by extraction of calcified mineral at low pH, neutralization with ammonium hydroxide, and colorimetric detection at 405 nm in a 96-well format. This assay is more sensitive than the cetylpyridinium chloride (CPC) extraction method, improving the detection of weakly mineralizing monolayers [1]. It provides a wider linear range: destained ARS dye ranging from 30 μM to 4 mM shows a linear relationship with the absorbance at 405 nm, making dilutions of samples prior to measurement unnecessary.

        We have applied this quantification assay to the osteogenesis induction of human mesenchymal stem cells (hMSCs). Cells were cultured in different osteogenic differentiation medium for 18 days, fixed for ARS staining and quantified for mineral deposit using the kit. There is significant difference between the untreated control, the induced samples, and the different induction conditions (Figure 2). ScienCell’s ARed-Q assay can be applied for mesenchymal stem cell osteogenic differentiation, tumor characterization, and osteognic compound screening.

        貨號8678
        產地美國
        縮寫ARed-Q
        規格100 Tests
        用途科研
        儲存室溫
        運輸膠冰
        No Publication available at this time
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        99久久香蕉国产线看观看
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