? ? ? 淋巴系統是免疫系統的重要部分,與血管系統共同作用來保持組織的內環境穩定。淋巴系統從組織中得到液體和大分子物質并將其返回血液循環,因此,淋巴系統在調節組織中液體,蛋白質和壓力平衡方面起著重要作用。盡管淋巴毛細管內皮細胞與血管內皮有許多相似的特性,但由于結構不同,又各具特點。淋巴毛細管缺少壁細胞且以基底膜不完整或缺失為特征。淋巴內皮的特征是包含大量的內褶和細胞質囊泡,并且具有交錯的細胞間連接。淋巴毛細管最顯著的特征是與間質的一體化,間質通過細微的彈性纖維與細胞外基質相連。
? ? ? ScienCell淋巴內皮細胞提取于人淋巴結組織,第一代凍存。每管含有細胞數>5×10^5cells/ml,此細胞通過vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗證,經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到15倍增。?
推薦培養基:內皮細胞培養基(ECM, Cat. #1001)
貨號 | 2500 |
產地 | 美國 |
縮寫 | HLEC |
規格 | 5 x 10^5 /1ml |
用途 | 科研 |
儲存 | 液氮 |
運輸 | 干冰 |
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Question:
Can you tell me the FVIII antibody used to test these cells. What company and catalogue # please.
Answers:
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Posted by Karishma Shah on Tuesday, October 18, 2016
Dear Colleen,
We use Anti-von Willebrand factor antibody (otherwise known as Factor
VIII related antigen) from Sigma-Aldrich. The catalog number is F3520.
Question:
Do these express podoplanin
Answers:
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Posted by Manoj Sharma on Thursday, January 14, 2016
Our quality control department does not test for podoplanin
expression in Human Lymphatic Endothelial Cells. We do test for
endothelial cell markers CD-31 and Factor VIII.