? ? ? 人黑色素細胞是神經嵴衍生細胞，位于人的某些器官，如皮膚、眼睛、內耳和軟腦膜。黑色素細胞不能向這些器官的移動，可以解釋先天性皮膚白斑(斑駁病)跟虹膜異色(不同顏色并置)之間的關系以及Waardenburg綜合征的先天性耳聾。皮膚黑色素細胞合成并轉運黑色素到周圍的角質細胞，使皮膚有色素沉積以保護皮膚對抗陽光輻射。隨著新的細胞培養技術和改良的培養方法，在成功培養這類細胞在純種群時，發現了新型的黑色素細胞基因——msgl，它能編碼一種與色素沉著相關的核蛋白。
? ? ? 人表皮黑色素細胞(深色素)提取于人表皮組織，一代凍存。每管含有細胞數>5x10^5cells/ml，此細胞通過Fibronectin和NGF-receptor(p75)免疫熒光染色驗證，經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到15倍增。
推薦培養基：黑色素細胞培養基 (MelM, Cat. #2201)
| 貨號 | 2230 |
| 產地 | 美國 |
| 縮寫 | HEM-a |
| 規格 | 5 x 10^5 /1ml |
| 用途 | 科研 |
| 儲存 | 液氮 |
| 運輸 | 干冰 |
1.) Nicholas C, Yang J, Peters SB, Bill MA, Baiocchi RA, Yan F, S?f S, Tae S, Gaudio E, Wu X, Grever MR, Young GS, and Lesinski1 GB. (2013) "PRMT5 Is Upregulated in Malignant and Metastatic Melanoma and Regulates Expression of MITF and p27Kip1."?PLoS One. 8: e74710.
2.) Looi CY, Moharram B, Paydar M, Wong YL, Leong KH, Mohamad K, Arya A, Wong WF, Mustafa MR. (2013) "Induction of apoptosis in melanoma A375 cells by a chloroform fraction of Centratherum anthelminticum (L.) seeds involves NF-kappaB, p53 and Bcl-2-controlled mitochondrial signaling pathways."?BMC Complement Altern Med. 13: 166.
3.) Rotte A, Bhandaru M, Cheng Y, Sjoestroem C, Martinka M, and Li G. (2013) "Decreased Expression of Nuclear p300 Is Associated with Disease Progression and Worse Prognosis of Melanoma Patients."?PLoS One. 8: e75405.
Question:
I purchased a HEM-a cell in February 2014 from ScienCell. Can you supply DNA (STR) profiling of this primary cell?
Thanks.
Answers:
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Posted by Manoj Sharma on Thursday, January 7, 2016
Hello,
We do not have STR profiling. thanks for your interest
SCIENCELL
