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ScienCell's GeneQuery? Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:

• M1 microglia phenotype markers: TNF, NOS2, IL1B
• M2 microglia phenotype markers: IL10, ARG1, MRC1
• Pericyte markers: ACTA2, PDGFRB, CSPG4
• Evaluation of neural differentiation
  • Astrocyte markers:   GFAP, S100B, GLUL
  • Immature neuron markers:   NEUROD1, DCX, TUBB3
  • Mature neuron markers:   MAP2, RBFOX3, SYP
• Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
• Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1

Note : all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

GeneQuery? Human Brain Development Markers qPCR Array Plate Layout*

(8 controls in Bold and Italic)

Note : all gene names follow their official symbols by HGNC

 * gene selection may be updated based on new research and development