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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人腦發育標記物qPCR檢測試劑盒

      英文名:Human Brain Development Markers qPCR Array Kit

      貨號:GK127

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Brain Development Markers qPCR Array Kit (GQH-BDM) surveys a panel of 24 marker genes involved in brain development, function and pathogenesis. GQH-BDM is also useful for evaluating the roles of stem and progenitor cells in brain organoid generation. Brief examples of how genes may be grouped are shown below:

      • M1 microglia phenotype markers: TNF, NOS2, IL1B
      • M2 microglia phenotype markers: IL10, ARG1, MRC1
      • Pericyte markers: ACTA2, PDGFRB, CSPG4
      • Evaluation of neural differentiation
        • Astrocyte markers:   GFAP, S100B, GLUL
        • Immature neuron markers:   NEUROD1, DCX, TUBB3
        • Mature neuron markers:   MAP2, RBFOX3, SYP
      • Neuron function evaluation: CHAT, TH (tyrosine hydroxylase), MAPT
      • Blood-brain barrier (BBB) evaluation: VCAM1, OCLN, ICAM1

      Note all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg 2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

       

      GeneQuery? Human Brain Development Markers qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

      Note : all gene names follow their official symbols by HGNC

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      TNF

      NOS2

      IL1B

      ACTB

      TNF

      NOS2

      IL1B

      ACTB

      TNF

      NOS2

      IL1B

      ACTB

      B

      IL10

      ARG1

      MRC1

      GAPDH

      IL10

      ARG1

      MRC1

      GAPDH

      IL10

      ARG1

      MRC1

      GAPDH

      C

      ACTA2

      PDGFRB

      CSPG4

      LDHA

      ACTA2

      PDGFRB

      CSPG4

      LDHA

      ACTA2

      PDGFRB

      CSPG4

      LDHA

      D

      GFAP

      S100B

      GLUL

      NONO

      GFAP

      S100B

      GLUL

      NONO

      GFAP

      S100B

      GLUL

      NONO

      E

      NEUROD1

      DCX

      TUBB3

      PPIH

      NEUROD1

      DCX

      TUBB3

      PPIH

      NEUROD1

      DCX

      TUBB3

      PPIH

      F

      MAP2

      RBFOX3

      SYP

      GDC

      MAP2

      RBFOX3

      SYP

      GDC

      MAP2

      RBFOX3

      SYP

      GDC

      G

      CHAT

      TH

      MAPT

      PPC

      CHAT

      TH

      MAPT

      PPC

      CHAT

      TH

      MAPT

      PPC

      H

      VCAM1

      OCLN

      ICAM1

      NTC

      VCAM1

      OCLN

      ICAM1

      NTC

      VCAM1

      OCLN

      ICAM1

      NTC

       * gene selection may be updated based on new research and development

      貨號GK127
      產地美國
      縮寫GQH-BDM
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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