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      人類轉錄因子qPCR檢測試劑盒

      英文名:Human Transcription Factors qPCR Array Kit

      貨號:GK124

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Transcription Factors qPCR Array Kit (GQH-TRF) surveys a panel of 88 transcription factors. Transcription factors are proteins that can regulate target gene transcription level by binding to specific regions of genome known as enhancers or silencers. Misregulation of transcription factors can lead to aberrations in signaling transduction and a variety of disorders including autoimmunity, neurological disorders, diabetes, cardiovascular disease and cancer. Brief examples of how genes may be grouped according to their functions are shown below:     

      • Stem cell-related: NANOG, POU5F1, SOX2, EGR1, FOXD3, FOXO1, KLF4, MEF2B, PAX6, SOX18
      • Cancer stem cell-related: NANOG, POU5F1, SOX2, AR, FOXO3, MYC, NKX3-1, SNAI1, SOX9, TFAP2A, TP53, TWIST1
      • Oxidative stress-related: ARNT, ETS1/2, IRF1, JUN, NFAT5, NFKB1, NFYA, SMAD1, SRF, STAT3
      • Neuroprotective function-related: CREB1, ESR1, FOXA1, GATA1/2/3, NFE2L2, NR4A2, PPARA, SP1, TBX3
      • Growth factor-mediated signaling pathways: CREBBP, ELK1, FOS, HIF1A, ID1, NFAT5, NFATs, STAT1/3/5A
      • Wnt signaling pathway: CEBPA, CEBPB, GBX2, HNF4A, LEF1, MITF, OLIG1, PITX2, SMAD4, SOX2
      • MAPK signaling pathway: ATF2/4, ELK1, FOX, JUN, MAX, MEF2A/C, NFKB1, SP1, STAT1/3
      • B/T-cell receptor signaling pathways: E2F1, ELK1, FOS, GATA3, JUN, MYB, NFATs, RUNX1, STAT6
      • Oncogenes & tumor suppressors: ABL1, BRCA1, CDKN2A, JUNB, MYCN, NF1, RARA, RB1, TGFB1, TNF

      Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

       

      GeneQuery? Human Transcription Factors qPCR Array Plate Layout *

      (8 controls in Bold and Italic)

      Noteall gene names follow their official symbols by HGNC

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ABL1

      CEBPB

      ETS1

      GATA2

      IRF1

      MEF2C

      NFATC1

      NR4A2

      REL

      SOX18

      STAT6

      ACTB

      B

      AR

      CREB1

      ETS2

      GATA3

      JUN

      MITF

      NFATC2

      OLIG1

      RELA

      SOX2

      TBX3

      GAPDH

      C

      ARNT

      CREBBP

      FOS

      GBX2

      JUNB

      MYB

      NFATC3

      PAX6

      RUNX1

      SOX9

      TFAP2A

      LDHA

      D

      ATF2

      CTNNB1

      FOXA1

      GLI1

      KLF4

      MYC

      NFATC4

      PITX2

      SMAD1

      SP1

      TGFB1

      NONO

      E

      ATF4

      E2F1

      FOXD3

      HIF1A

      LEF1

      MYCN

      NFE2L2

      POU5F1

      SMAD4

      SRF

      TNF

      PPIH

      F

      BRCA1

      EGR1

      FOXO1

      HNF4A

      MAX

      NANOG

      NFKB1

      PPARA

      SMAD5

      STAT1

      TP53

      GDC

      G

      CDKN2A

      ELK1

      FOXO3

      HSF1

      MEF2A

      NF1

      NFYA

      RARA

      SMAD9

      STAT3

      TWIST1

      PPC

      H

      CEBPA

      ESR1

      GATA1

      ID1

      MEF2B

      NFAT5

      NKX3-1

      RB1

      SNAI1

      STAT5A

      WT1

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK124
      產地美國
      縮寫GQH-TRF
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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