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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人胸腺細胞發育標記qPCR檢測試劑盒

      英文名:Human Thymocyte Development Markers qPCR Array Kit

      貨號:GK117

      價格:$250

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      ScienCell's GeneQuery? Human Thymocyte Development Markers qPCR Array Kit (GQH-TDM) is designed to facilitate gene expression profiling of 40 marker genes involved in thymopoiesis.  The tightly regulated lineages of common lymphoid progenitors (CLPs) consist of several maturational stages based on the expression of cell surface markers, including double negative stage (CD4- and CD8-), double positive stage (CD4+ and CD8+), and single positive stage (CD4+ or CD8+). The CD4+ T cells can further differentiate into a number of effectors subsets such as Th1, Th2, Th9, Th17, and Tfh. Brief examples of how included genes may be grouped according to their functions are shown below:

      Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

      • CLP markers: MME, CD34, PTPRC, IL7R, FLT3
      • Double negative thymocyte markers: CD1A, CD3D, CD3E, CD3G, CD5, CD7, CD200, IL2RA
      • Double positive thymocyte markers: CD1A, CD4, CD5, CD7, CD8A, CD8B, CD38, SELL, CD200
      • CD4+ T cell markers: CD3D, CD3E, CD3G, CD4, PTPRC, IL2RA, SELL
      • CD8+ T cell markers: CD3D, CD3E, CD3G, CD8A, CD8B, GZMB
      • Regulatory T cell markers: IL2RA, ENTPD1, NT5E, ITGAE, FOXP3
      • Markers of T cell effector subsets
        • Th1: FASLG, CXCR3, CCR5, IL12RB1, IL18R1
        • Th2: CXCR3, CCR3, CCR4, CCR8, PTGDR2
        • Th9: CCR6, IL9
        • Th17: PTPRC, IL6R, KLRB1, CCR4, CCR6, IL13RA1, IL21R
        • Tfh: CXCR5, PDCD1                                              

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human Thymocyte Development Markers qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

      Note: all gene names follow their official symbols by HGNC

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      CCR3

      CD38

      CD8B

      IL12RB1

      ITGAE

      ACTB

      CCR3

      CD38

      CD8B

      IL12RB1

      ITGAE

      ACTB

      B

      CCR4

      CD3D

      CXCR3

      IL13RA1

      KLRB1

      GAPDH

      CCR4

      CD3D

      CXCR3

      IL13RA1

      KLRB1

      GAPDH

      C

      CCR5

      CD3E

      CXCR5

      IL18R1

      MME

      LDHA

      CCR5

      CD3E

      CXCR5

      IL18R1

      MME

      LDHA

      D

      CCR6

      CD3G

      ENTPD1

      IL21R

      NT5E

      NONO

      CCR6

      CD3G

      ENTPD1

      IL21R

      NT5E

      NONO

      E

      CCR8

      CD4

      FASLG

      IL2RA

      PDCD1

      PPIH

      CCR8

      CD4

      FASLG

      IL2RA

      PDCD1

      PPIH

      F

      CD1A

      CD5

      FLT3

      IL6R

      PTGDR2

      GDC

      CD1A

      CD5

      FLT3

      IL6R

      PTGDR2

      GDC

      G

      CD200

      CD7

      FOXP3

      IL7R

      PTPRC

      PPC

      CD200

      CD7

      FOXP3

      IL7R

      PTPRC

      PPC

      H

      CD34

      CD8A

      GZMB

      IL9

      SELL

      NTC

      CD34

      CD8A

      GZMB

      IL9

      SELL

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK117
      產地美國
      縮寫GQH-TDM
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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