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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        人類骨髓譜系標記qPCR檢測試劑盒

        英文名:Human Myeloid Lineage Markers qPCR Array Kit

        貨號:GK115

        價格:$250

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        ScienCell's GeneQuery? Human Myeloid Lineage Markers qPCR Array Kit (GQH-MLM) is designed to facilitate gene expression profiling of 40 marker genes involved in myelopoiesis. In the tightly regulated lineage of common myeloid progenitors (CMPs), the CMPs can first differentiate into granulocytes, monocytes, mast cells and macrophages. The granulocytes can further differentiate into mature eosinophils, basophils, and neutrophils. Brief examples of how included genes may be grouped according to their functions are shown below:     

        • CMP markers: CD34, CD38, KIT, FLT3
        • Granulocyte-monocyte precursor markers: CD34, CD38, CD48, IL3RA, FLT3
        • Monocyte markers: ITGAM, CD14, PECAM1, SELL, CSF1R, CCR2, FCGR3A, FCGR3B, CXCR3
        • Macrophage markers: ITGAM, ITGAX, CD14, CD68, CD80, CD163
        • Polarized macrophage markers: CD68, CD86, TLR2, TLR4, CIITA, CD163, CD200R1, MRC1, CD81, VCAM1, CLEC7A, VEGFA
        • Mast cell markers: FCER2, KIT, ENPP3, FCER1A
        • Neutrophil markers: ANPEP, FUT4, CD33
        • Eosinophil markers: CD9, FCER2, FCGR2A, FCGR2B, FCGR2C
        • Basophil markers: CD69, IL3RA, FCER1A

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

        GeneQuery? Human Myeloid Lineage Markers qPCR Array Plate Layout*

        (8 controls in Bold and Italic)

         

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ANPEP

        CD48

        CLEC7A

        FCGR2C

        KIT

        β-actin

        ANPEP

        CD48

        CLEC7A

        FCGR2C

        KIT

        β-actin

        B

        CCR2

        CD68

        CSF1R

        FCGR3A

        MRC1

        GAPDH

        CCR2

        CD68

        CSF1R

        FCGR3A

        MRC1

        GAPDH

        C

        CD14

        CD69

        CXCR3

        FCGR3B

        PECAM1

        LDHA

        CD14

        CD69

        CXCR3

        FCGR3B

        PECAM1

        LDHA

        D

        CD163

        CD80

        ENPP3

        FLT3

        SELL

        NONO

        CD163

        CD80

        ENPP3

        FLT3

        SELL

        NONO

        E

        CD200R1

        CD81

        FCER1A

        FUT4

        TLR2

        PPIH

        CD200R1

        CD81

        FCER1A

        FUT4

        TLR2

        PPIH

        F

        CD33

        CD86

        FCER2

        IL3RA

        TLR4

        GDC

        CD33

        CD86

        FCER2

        IL3RA

        TLR4

        GDC

        G

        CD34

        CD9

        FCGR2A

        ITGAM

        VCAM1

        PPC

        CD34

        CD9

        FCGR2A

        ITGAM

        VCAM1

        PPC

        H

        CD38

        CIITA

        FCGR2B

        ITGAX

        VEGFA

        NTC

        CD38

        CIITA

        FCGR2B

        ITGAX

        VEGFA

        NTC

         

        * gene selection may be updated based on new research and development

        貨號GK115
        產地美國
        縮寫GQH-MLM
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看

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