99久久香蕉国产线看观看

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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        人類小膠質細胞極化標記qPCR檢測試劑盒

        英文名:Human Microglial Polarization Markers qPCR Array Kit

        貨號:GK114

        價格:$250

        產品說明書下載:

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        ScienCell's GeneQuery? Human Microglial Polarization Markers qPCR Array Kit (GQH-MPM) is designed to facilitate gene expression profiling of 40 marker genes involved in microglial polarization, which has important implications for regulating innate immunological functions. When microglia are stimulated, two potential phenotypes can be observed: pro-inflammatory M1 microglia and anti-inflammatory M2 microglia. The M1 phenotype acts to protect tissues against invading pathogens by releasing cytokines and revoking acute inflammation, while the M2 phenotype acts to restore tissue homeostasis and stimulate tissue repair. Brief examples of how included genes may be grouped according to their functions are shown below:     

        • M1 phenotype marker: TNF, FCGR2A, FCGR2B, FCGR2C, FCGR3A, FCGR3B, CD86, IL1B, NOS2, IL6
        • M2 phenotype marker: MRC1, IL4, IL10, TGFB1, IL1R1, SOCS3, ARG1, IL13, SPHK1
        • Activation/resting markers: CD80, CLEC5A, HEXB, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, IRF8, ITGAM, MYB, PTGES, RUNX1, TLR3, TLR4
        • Additional microglia markers: ADGRE1, AIF1, CD40, CD68, EDA, ITGAM, ITGAX, PTPRC

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

        GeneQuery? Human Microglial Polarization Markers qPCR Array Plate Layout*

        (8 controls in Bold and Italic)

         

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ADGRE1

        EDA

        HLA-DQB1

        IL6

        PTPRC

        β-actin

        ADGRE1

        EDA

        HLA-DQB1

        IL6

        PTPRC

        β-actin

        B

        AIF1

        FCGR2A

        HLA-DRA

        IRF8

        RUNX1

        GAPDH

        AIF1

        FCGR2A

        HLA-DRA

        IRF8

        RUNX1

        GAPDH

        C

        ARG1

        FCGR2B

        HLA-DRB1

        ITGAM

        SOCS3

        LDHA

        ARG1

        FCGR2B

        HLA-DRB1

        ITGAM

        SOCS3

        LDHA

        D

        CD40

        FCGR2C

        IL10

        ITGAX

        SPHK1

        NONO

        CD40

        FCGR2C

        IL10

        ITGAX

        SPHK1

        NONO

        E

        CD68

        FCGR3A

        IL13

        MRC1

        TGFB1

        PPIH

        CD68

        FCGR3A

        IL13

        MRC1

        TGFB1

        PPIH

        F

        CD80

        FCGR3B

        IL1B

        MYB

        TLR3

        GDC

        CD80

        FCGR3B

        IL1B

        MYB

        TLR3

        GDC

        G

        CD86

        HEXB

        IL1R1

        NOS2

        TLR4

        PPC

        CD86

        HEXB

        IL1R1

        NOS2

        TLR4

        PPC

        H

        CLEC5A

        HLA-DQA1

        IL4

        PTGES

        TNF

        NTC

        CLEC5A

        HLA-DQA1

        IL4

        PTGES

        TNF

        NTC

         

        * gene selection may be updated based on new research and development

        貨號GK114
        產地美國
        縮寫GQH-MPM
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看

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