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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類小膠質細胞極化標記qPCR檢測試劑盒

      英文名:Human Microglial Polarization Markers qPCR Array Kit

      貨號:GK114

      價格:$250

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      ScienCell's GeneQuery? Human Microglial Polarization Markers qPCR Array Kit (GQH-MPM) is designed to facilitate gene expression profiling of 40 marker genes involved in microglial polarization, which has important implications for regulating innate immunological functions. When microglia are stimulated, two potential phenotypes can be observed: pro-inflammatory M1 microglia and anti-inflammatory M2 microglia. The M1 phenotype acts to protect tissues against invading pathogens by releasing cytokines and revoking acute inflammation, while the M2 phenotype acts to restore tissue homeostasis and stimulate tissue repair. Brief examples of how included genes may be grouped according to their functions are shown below:     

      • M1 phenotype marker: TNF, FCGR2A, FCGR2B, FCGR2C, FCGR3A, FCGR3B, CD86, IL1B, NOS2, IL6
      • M2 phenotype marker: MRC1, IL4, IL10, TGFB1, IL1R1, SOCS3, ARG1, IL13, SPHK1
      • Activation/resting markers: CD80, CLEC5A, HEXB, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1, IRF8, ITGAM, MYB, PTGES, RUNX1, TLR3, TLR4
      • Additional microglia markers: ADGRE1, AIF1, CD40, CD68, EDA, ITGAM, ITGAX, PTPRC

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human Microglial Polarization Markers qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ADGRE1

      EDA

      HLA-DQB1

      IL6

      PTPRC

      β-actin

      ADGRE1

      EDA

      HLA-DQB1

      IL6

      PTPRC

      β-actin

      B

      AIF1

      FCGR2A

      HLA-DRA

      IRF8

      RUNX1

      GAPDH

      AIF1

      FCGR2A

      HLA-DRA

      IRF8

      RUNX1

      GAPDH

      C

      ARG1

      FCGR2B

      HLA-DRB1

      ITGAM

      SOCS3

      LDHA

      ARG1

      FCGR2B

      HLA-DRB1

      ITGAM

      SOCS3

      LDHA

      D

      CD40

      FCGR2C

      IL10

      ITGAX

      SPHK1

      NONO

      CD40

      FCGR2C

      IL10

      ITGAX

      SPHK1

      NONO

      E

      CD68

      FCGR3A

      IL13

      MRC1

      TGFB1

      PPIH

      CD68

      FCGR3A

      IL13

      MRC1

      TGFB1

      PPIH

      F

      CD80

      FCGR3B

      IL1B

      MYB

      TLR3

      GDC

      CD80

      FCGR3B

      IL1B

      MYB

      TLR3

      GDC

      G

      CD86

      HEXB

      IL1R1

      NOS2

      TLR4

      PPC

      CD86

      HEXB

      IL1R1

      NOS2

      TLR4

      PPC

      H

      CLEC5A

      HLA-DQA1

      IL4

      PTGES

      TNF

      NTC

      CLEC5A

      HLA-DQA1

      IL4

      PTGES

      TNF

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK114
      產地美國
      縮寫GQH-MPM
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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