99久久香蕉国产线看观看

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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人毛乳頭細胞 HHDPC

      英文名:Human Hair Dermal Papilla Cells

      貨號:2400

      價格:$738

      產品說明書下載:

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      ? ? ? 毛乳頭是細胞中高活性的團體。它來源于真皮的間葉細胞,位于毛囊的基部。毛乳頭能控制頭發生長周期,誘導表皮毛囊發育并生成毛發纖維。培養早期,毛乳頭細胞能誘導毛發體外生長,但進一步培養,該特性消失。毛發生長受到毛囊細胞與間葉上皮細胞間相互作用的調節。例如:毛乳頭細胞分泌的β干擾素抑制體外培養中外根鞘細胞的生長。毛乳頭細胞的存在也受到信號轉導途徑的影響,激活的ERK和Akt促進毛乳頭細胞的存活。


      ? ? ? ScienCell人毛乳頭細胞提取于人毛乳頭組織,一代凍存。每管含有細胞數>5x10^5cells/ml,此細胞通過纖維粘連蛋白和CD105免疫熒光染色驗證,經測試不含有HIV-1、HBV、HCV、支原體、細菌、酵母和真菌。細胞可以達到15倍增。

      推薦培養基:間充質干細胞培養基 (MSCM, Cat. #7501)

      貨號2400
      產地美國
      縮寫HHDPC
      規格5 x 10^5 /1ml
      用途科研
      儲存液氮
      運輸干冰
      [1] Filsell, W., Little, J.C., Stones, A.J., Granger, S.P. and Bayley, S.A. (1994) Transfection of rat dermal papilla cells with a gene encoding a temperature-sensitive polyomavirus large T antigen generates cell lines retaining a differentiated phenotype. Journal of Cell Science 107:1761-1772.?

      [2] Kim, C.D., Choe, Y., Shim, C., Kim, K. (2002) Interferon Beta secreted from human hair dermal papilla cells inhibits the growth of outer root sheath cells cultured in vitro. Biochem Biophys Res Commun. 290:1133-8.?

      [3] Han, J.H., Kwon, O.S., Chung, J.H., Cho, K.H., Eun, H.C., Kim, K.H. (2004) Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J Dermatol Sci. 34:91-8.

      Question:

      Hi, I saw the subculturing method and I understood about poly L lysine coating for maintaining the cells. And I'm wondering if I have to do coating with poly L lysine even in a 96 well plate for some kinds of assays. Without the coating, is it going to be affected to the cells?

      Answers:

      • Posted by Karishma Shah on Thursday, October 20, 2016

        Dear customer,

        Yes, poly-l-lysine must be used to, especially for a 96-well plate. Primary cells generally need a substrate to attach to and the TC coating on the plate or flask is usually not sufficient. Sometimes attachment on 96 –well plates can be challenging, so we would definitely recommend using poly-l-lysine.

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      99久久香蕉国产线看观看

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