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        人內皮素信號qPCR檢測試劑盒

        英文名:Human Endothelin Signaling qPCR Array Kit

        貨號:GK107

        價格:$250

        產品說明書下載:

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        ScienCell's GeneQuery? Human Endothelin Signaling qPCR Array Kit (GQH-EDN) surveys 88 key genes involved in the endothelin signaling transduction with a particular focus on endothelin-1.  Endothelins are secreted proteins that regulate vasoconstriction and are produced primarily in the endothelium.  Endothelin receptors are also present in cells of the central nervous system and abnormal signaling may be involved in seizures and peripheral neuropathy.  Other diseases implicated in aberrant endothelin signaling include cardiovascular disorders, vasospasm, cardiac hypertrophy, and Dengue fever.  Brief examples of how included genes may be categorized are shown below:

        • Calcium regulation: CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D
        • Cytoskeletal remodeling: CAPN2, MAP2K1, PLD1, PLD2, RHOA
        • Cell cycling regulation: CCND3, CDC42, ITPR1, ITPR3, RIPK1
        • Transcription factors: ELK1, FOS, JUN, MYC, SP1
        • RAS superfamily GTPases: HRAS, KRAS, NRAS, RAC1, RAP1A

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

        GeneQuery? Human Endothelin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)

         

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ADCY1

        CACNA1D

        CDC42

        EDNRA

        GSK3B

        MAP2K1

        MYC

        PIK3R1

        PPP1CA

        PTGS2

        RIPK1

        β-actin

        B

        ADCY3

        CALM1

        CGA

        EDNRB

        HBEGF

        MAP3K1

        NOS3

        PIK3R2

        PPP3CC

        PTK2B

        SHC1

        GAPDH

        C

        ADCY8

        CALM2

        CRK

        EGFR

        HRAS

        MAPK13

        NPPB

        PIK3R3

        PRKCA

        RAC1

        SOS1

        LDHA

        D

        AKT1

        CAMK2A

        DGKB

        ELK1

        ITPR1

        MAPK14

        NRAS

        PLA2G4A

        PRKCD

        RAF1

        SOS2

        NONO

        E

        AKT2

        CAMK2B

        DGKE

        FOS

        ITPR2

        MAPK3

        NTRK1

        PLA2G7

        PRKCE

        RAP1A

        SP1

        PPIH

        F

        ASIC1

        CAMK2D

        ECE1

        GNAI1

        ITPR3

        MAPK9

        PIK3CA

        PLCG1

        PTGER2

        RAPGEF1

        SRC

        GDC

        G

        ATF4

        CAPN2

        EDN1

        GNAQ

        JUN

        MMP2

        PIK3CB

        PLD1

        PTGER4

        RASGRP1

        STAT5A

        PPC

        H

        CACNA1C

        CCND3

        EDN3

        GRB2

        KRAS

        MT-CO2

        PIK3CD

        PLD2

        PTGS1

        RHOA

        TRPV1

        NTC

         

         * gene selection may be updated based on new research and development

        貨號GK107
        產地美國
        縮寫GQH-EDN
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看
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