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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人內皮素信號qPCR檢測試劑盒

      英文名:Human Endothelin Signaling qPCR Array Kit

      貨號:GK107

      價格:$250

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      ScienCell's GeneQuery? Human Endothelin Signaling qPCR Array Kit (GQH-EDN) surveys 88 key genes involved in the endothelin signaling transduction with a particular focus on endothelin-1.  Endothelins are secreted proteins that regulate vasoconstriction and are produced primarily in the endothelium.  Endothelin receptors are also present in cells of the central nervous system and abnormal signaling may be involved in seizures and peripheral neuropathy.  Other diseases implicated in aberrant endothelin signaling include cardiovascular disorders, vasospasm, cardiac hypertrophy, and Dengue fever.  Brief examples of how included genes may be categorized are shown below:

      • Calcium regulation: CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D
      • Cytoskeletal remodeling: CAPN2, MAP2K1, PLD1, PLD2, RHOA
      • Cell cycling regulation: CCND3, CDC42, ITPR1, ITPR3, RIPK1
      • Transcription factors: ELK1, FOS, JUN, MYC, SP1
      • RAS superfamily GTPases: HRAS, KRAS, NRAS, RAC1, RAP1A

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Endothelin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ADCY1

      CACNA1D

      CDC42

      EDNRA

      GSK3B

      MAP2K1

      MYC

      PIK3R1

      PPP1CA

      PTGS2

      RIPK1

      β-actin

      B

      ADCY3

      CALM1

      CGA

      EDNRB

      HBEGF

      MAP3K1

      NOS3

      PIK3R2

      PPP3CC

      PTK2B

      SHC1

      GAPDH

      C

      ADCY8

      CALM2

      CRK

      EGFR

      HRAS

      MAPK13

      NPPB

      PIK3R3

      PRKCA

      RAC1

      SOS1

      LDHA

      D

      AKT1

      CAMK2A

      DGKB

      ELK1

      ITPR1

      MAPK14

      NRAS

      PLA2G4A

      PRKCD

      RAF1

      SOS2

      NONO

      E

      AKT2

      CAMK2B

      DGKE

      FOS

      ITPR2

      MAPK3

      NTRK1

      PLA2G7

      PRKCE

      RAP1A

      SP1

      PPIH

      F

      ASIC1

      CAMK2D

      ECE1

      GNAI1

      ITPR3

      MAPK9

      PIK3CA

      PLCG1

      PTGER2

      RAPGEF1

      SRC

      GDC

      G

      ATF4

      CAPN2

      EDN1

      GNAQ

      JUN

      MMP2

      PIK3CB

      PLD1

      PTGER4

      RASGRP1

      STAT5A

      PPC

      H

      CACNA1C

      CCND3

      EDN3

      GRB2

      KRAS

      MT-CO2

      PIK3CD

      PLD2

      PTGS1

      RHOA

      TRPV1

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK107
      產地美國
      縮寫GQH-EDN
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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