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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類焦點粘附復合物qPCR檢測試劑盒

      英文名:Human Focal Adhesion Complexes qPCR Array Kit

      貨號:GK106

      價格:$250

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      ScienCell's GeneQuery? Human Focal Adhesion Complexes qPCR Array Kit (GQH-FAC) profiles 88 key genes involved in the regulation and formation of focal adhesions.  Focal adhesions are large macromolecular complexes by which the cell interacts with its extracellular matrix (ECM) environment.  They are involved in such diverse processes as interpreting mechanical force, regulating cell adhesion and motility, and contributing to intracellular signaling transduction.  Integrins and focal adhesion kinase (PTK2) are the main regulatory elements of focal adhesions.  Brief examples of how included genes may be categorized are shown below:

      • FAK signaling: CCND1, FYN, PTK2, RHOA, SRC
      • Cytoskeletal elements: ACTB, ACTN1, ACTN3, FLNA, VCL
      • ECM components: COL1A2, COL2A1, COL4A2, FN1, LAMC3
      • ECM binding elements: ITGA1, ITGA5, ITGAM, ITGB1, ITGB3
      • Signaling transduction genes: ARHGAP5, ELK1, HGF, JUN, KDR

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Focal Adhesion Complexes qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACTB

      BRAF

      COL4A2

      FYN

      ITGA3

      ITGAL

      ITGB5

      MAP2K1

      PAK2

      PXN

      SHC1

      β-actin

      B

      ACTN1

      CAV1

      CRK

      GRB2

      ITGA4

      ITGAM

      ITGB6

      MAPK1

      PAK4

      RAC1

      SHC3

      GAPDH

      C

      ACTN3

      CAV2

      DOCK1

      HGF

      ITGA5

      ITGAV

      ITGB7

      MAPK10

      PDGFRA

      RAF1

      SOS1

      LDHA

      D

      AKT1

      CAV3

      EGFR

      HRAS

      ITGA6

      ITGAX

      JUN

      MAPK8

      PDGFRB

      RAP1A

      SRC

      NONO

      E

      AKT2

      CCND1

      ELK1

      ILK

      ITGA7

      ITGB1

      KDR

      MAPK9

      PDPK1

      RAPGEF1

      TLN1

      PPIH

      F

      AKT3

      CDC42

      FLNA

      ITGA1

      ITGA8

      ITGB2

      LAMA1

      MET

      PIK3R1

      RHOA

      VAV3

      GDC

      G

      ARHGAP5

      COL1A2

      FLT1

      ITGA2

      ITGAD

      ITGB3

      LAMB1

      MYLK2

      PIK3R2

      ROCK1

      VCL

      PPC

      H

      BCAR1

      COL2A1

      FN1

      ITGA2B

      ITGAE

      ITGB4

      LAMC3

      PAK1

      PTK2

      ROCK2

      VTN

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK106
      產地美國
      縮寫GQH-FAC
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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