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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        人類焦點粘附復合物qPCR檢測試劑盒

        英文名:Human Focal Adhesion Complexes qPCR Array Kit

        貨號:GK106

        價格:$250

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        ScienCell's GeneQuery? Human Focal Adhesion Complexes qPCR Array Kit (GQH-FAC) profiles 88 key genes involved in the regulation and formation of focal adhesions.  Focal adhesions are large macromolecular complexes by which the cell interacts with its extracellular matrix (ECM) environment.  They are involved in such diverse processes as interpreting mechanical force, regulating cell adhesion and motility, and contributing to intracellular signaling transduction.  Integrins and focal adhesion kinase (PTK2) are the main regulatory elements of focal adhesions.  Brief examples of how included genes may be categorized are shown below:

        • FAK signaling: CCND1, FYN, PTK2, RHOA, SRC
        • Cytoskeletal elements: ACTB, ACTN1, ACTN3, FLNA, VCL
        • ECM components: COL1A2, COL2A1, COL4A2, FN1, LAMC3
        • ECM binding elements: ITGA1, ITGA5, ITGAM, ITGB1, ITGB3
        • Signaling transduction genes: ARHGAP5, ELK1, HGF, JUN, KDR

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

        GeneQuery? Human Focal Adhesion Complexes qPCR Array Plate Layout* (8 controls in Bold and Italic)

         

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ACTB

        BRAF

        COL4A2

        FYN

        ITGA3

        ITGAL

        ITGB5

        MAP2K1

        PAK2

        PXN

        SHC1

        β-actin

        B

        ACTN1

        CAV1

        CRK

        GRB2

        ITGA4

        ITGAM

        ITGB6

        MAPK1

        PAK4

        RAC1

        SHC3

        GAPDH

        C

        ACTN3

        CAV2

        DOCK1

        HGF

        ITGA5

        ITGAV

        ITGB7

        MAPK10

        PDGFRA

        RAF1

        SOS1

        LDHA

        D

        AKT1

        CAV3

        EGFR

        HRAS

        ITGA6

        ITGAX

        JUN

        MAPK8

        PDGFRB

        RAP1A

        SRC

        NONO

        E

        AKT2

        CCND1

        ELK1

        ILK

        ITGA7

        ITGB1

        KDR

        MAPK9

        PDPK1

        RAPGEF1

        TLN1

        PPIH

        F

        AKT3

        CDC42

        FLNA

        ITGA1

        ITGA8

        ITGB2

        LAMA1

        MET

        PIK3R1

        RHOA

        VAV3

        GDC

        G

        ARHGAP5

        COL1A2

        FLT1

        ITGA2

        ITGAD

        ITGB3

        LAMB1

        MYLK2

        PIK3R2

        ROCK1

        VCL

        PPC

        H

        BCAR1

        COL2A1

        FN1

        ITGA2B

        ITGAE

        ITGB4

        LAMC3

        PAK1

        PTK2

        ROCK2

        VTN

        NTC

         

         * gene selection may be updated based on new research and development

        貨號GK106
        產地美國
        縮寫GQH-FAC
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看
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