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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類ECM降解qPCR檢測試劑盒

      英文名:Human ECM Degradation qPCR Array Kit

      貨號:GK105

      價格:$250

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      ScienCell's GeneQuery? Human ECM Degradation qPCR Array Kit (GQH-ECM) profiles 88 key genes involved in extracellular matrix (ECM) degradation.  The ECM comprises molecules secreted by cells that provide structural and biochemical support.  Its roles include supporting intercellular communication, growth factor storage, and regulating cell migration and motility.  Degradation of the ECM thus influences growth factor release, chemotaxis, cell migration, and cellular adhesion.  Enzymes classically involved in ECM degradation include serine proteases, threonine proteases, and matrix metalloproteinases.  Brief examples of how included genes may be categorized are shown below:

      • ECM components: ACAN, COL18A1, COL4A1, FGG, LAMA5, NTN4
      • Proteases: ADAM10, ADAMTS1, BMP2, CAPN9, CTSG, MMP2, MMP9
      • Protease inhibitors: A2M, CAST, SERPINH1, TIMP1, TIMP2
      • ECM proteoglycans: BGN, DCN, LTBP2, LUM, PCOLCE
      • Cell adhesion receptors and glycoproteins: CD44, CEACAM8, FN1, HSPG2, SPARC

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

       

      GeneQuery? Human ECM Degradation qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      A2M

      ADAMTS4

      CAPN1

      CEACAM8

      COL4A1

      CTSG

      FGB

      LAMA2

      LOX

      MMP3

      PLG

      β-actin

      B

      ACAN

      ADAMTS5

      CAPN10

      COL11A1

      COL4A3

      CTSS

      FGG

      LAMA3

      LTBP2

      MMP7

      PSEN1

      GAPDH

      C

      ADAM10

      BGN

      CAPN2

      COL14A1

      COL4A5

      CTSV

      FN1

      LAMA5

      LUM

      MMP8

      SERPINH1

      LDHA

      D

      ADAM12

      BMP1

      CAPN3

      COL18A1

      COL5A1

      DCN

      HSPG2

      LAMB1

      MMP1

      MMP9

      SPARC

      NONO

      E

      ADAM15

      BMP10

      CAPN9

      COL1A1

      COL5A2

      DMD

      HTRA1

      LAMB2

      MMP12

      NCSTN

      SPP1

      PPIH

      F

      ADAM17

      BMP2

      CASP3

      COL1A2

      COL6A1

      DMP1

      KLK12

      LAMB3

      MMP13

      NID1

      TIMP1

      GDC

      G

      ADAM8

      BMP4

      CAST

      COL2A1

      COL6A3

      FBN1

      KLK7

      LAMC1

      MMP14

      NTN4

      TIMP2

      PPC

      H

      ADAMTS1

      BMP7

      CD44

      COL3A1

      COL7A1

      FGA

      LAMA1

      LAMC2

      MMP2

      PCOLCE

      VCAN

      NTC

       

       * gene selection may be updated based on new research and development

       

      貨號GK105
      產地美國
      縮寫GQH-ECM
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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