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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類細胞骨架重塑qPCR檢測試劑盒

      英文名:Human Cytoskeletal Remodeling qPCR Array Kit

      貨號:GK104

      價格:$250

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      ScienCell's GeneQuery? Human Cytoskeletal Remodeling qPCR Array Kit (GQH-CYT) profiles 88 key genes involved in cytoskeletal remodeling with a particular focus on Rho GTPase actin cytoskeleton remodeling.  The cytoskeleton is responsible for maintaining cell shape, scaffolding, and regulating intracellular transport.  It is also involved in numerous signaling pathways, cellular division, vesicular trafficking, and motility.  Microfilaments, which are primarily actin polymers, are part of the cytoskeleton and regulated by the Rho family of GTPases.  Brief examples of how included genes may be categorized are shown below:

      • Cytoskeletal components: ACTA1, ACTB, ACTG1, ACTN1, MYH10, MYL1
      • Integrins: ITGA1, ITGA3, ITGAV, ITGB1, ITGB3
      • Extracellular matrix components: COL1A1, COL2A1, COL4A1, LAMA1, LAMB1
      • Rho signaling molecules: CAV1, CDC42, RAC1, RHOA, ROCK1, ROCK2
      • Transcription factors: CDX1, CDX2, SP1, TCF7, TCF7L1

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Cytoskeletal Remodeling qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACTA1

      ACTN3

      CFL1

      COL4A1

      ITGA3

      LAMB1

      MYH2

      MYL12B

      MYLK

      PTK2

      SRC

      β-actin

      B

      ACTA2

      ACTN4

      CFL2

      COL4A2

      ITGA5

      LAMC1

      MYH3

      MYL2

      MYLK2

      PXN

      TCF7

      GAPDH

      C

      ACTB

      ACTR3B

      CLDN4

      COL4A5

      ITGA7

      LEF1

      MYH6

      MYL3

      MYLK3

      RAC1

      TCF7L1

      LDHA

      D

      ACTC1

      ARHGDIA

      CLDN5

      FLNA

      ITGA8

      LIMK1

      MYH7

      MYL4

      MYLPF

      RHOA

      TCF7L2

      NONO

      E

      ACTG1

      CAV1

      CLDN7

      FN1

      ITGAV

      MAPK1

      MYH7B

      MYL5

      NECTIN1

      ROCK1

      TJP1

      PPIH

      F

      ACTG2

      CDC42

      COL1A1

      GRB2

      ITGB1

      MAPK3

      MYH8

      MYL6

      NKX2-1

      ROCK2

      TLN1

      GDC

      G

      ACTN1

      CDX1

      COL1A2

      ITGA1

      ITGB3

      MYH10

      MYH9

      MYL6B

      PAK1

      SNAI1

      TLN2

      PPC

      H

      ACTN2

      CDX2

      COL2A1

      ITGA2

      LAMA1

      MYH11

      MYL1

      MYL9

      PIP5K1C

      SP1

      VCL

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK104
      產地美國
      縮寫GQH-CYT
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存 4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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