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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人角質形成細胞生物學qPCR檢測試劑盒

      英文名:Human Keratinocyte Cell Biology qPCR Array Kit

      貨號:GK100

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Keratinocyte Cell Biology qPCR Array Kit (GQH-KCB) is designed to facilitate gene expression profiling of 88 key genes involved in human keratinocyte cell biology, including keratinocyte proliferation and differentiation, extracellular matrix (ECM) synthesis, cell adhesion and epithelialization. Genes implicated in skin carcinogenesis and psoriasis are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

      • Keratinocyte cell markers: KRT10, KRT14, IVL, BNC1, TP63, KRTDAP
      • Proliferation and differentiation: KRT1/8/10/13/18, IVL, FLG, FLG2, LOR, TGM1, CDK1, CTNNB1, IL1B, CYR61
      • Migration and epithelialization: EGFR, HGF, MET, EGR3, AKAP12, PTGS2, LAMA3, LAMB3, LAMC2, FGFR1/2, IGF1, EGF
      • ECM synthesis: COL1A1/2, COL4A1/2, COL7A1, FN1, VTN, Laminins
      • Cell adhesion: CDH1/2/3/15, DSC1/2, DSG1/3, ITGA2, ITGB1, SELE/P, ICAM1/3, VCAM1, ITGA3/6, ITGB4
      • Skin carcinogenesis: PTCH1/2, SMO, TP53, GLI1/2, SHH, MC1R, CTNNB1, KRT15/17/20
      • Psoriasis: HLA-C, CDSN, IL36RN, TRAF3IP2, PSORS1C1/2, FLG, FABP5

      Noteall gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human Keratinocyte Cell Biology qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

      Noteall gene names follow their official symbols by HGNC

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      AKAP12

      CDSN

      DSC1

      FGFR1

      HLA-C

      ITGA2

      KRT1

      KRT20

      LAMC1

      PSORS1C2

      TGM1

      ACTB

      B

      BNC1

      COL1A1

      DSC2

      FGFR2

      ICAM1

      ITGA3

      KRT10

      KRT8

      LAMC2

      PTCH1

      TP53

      GAPDH

      C

      CCL2

      COL1A2

      DSG1

      FLG

      ICAM3

      ITGA6

      KRT13

      KRTDAP

      LOR

      PTCH2

      TP63

      LDHA

      D

      CDH1

      COL4A1

      DSG3

      FLG2

      IGF1

      ITGB1

      KRT14

      LAMA1

      MC1R

      PTGS2

      TRAF3IP2

      NONO

      E

      CDH15

      COL4A2

      EGF

      FN1

      IGF1R

      ITGB1BP1

      KRT15

      LAMA3

      MET

      SELE

      VCAM1

      PPIH

      F

      CDH2

      COL7A1

      EGFR

      GLI1

      IL1B

      ITGB4

      KRT16

      LAMB1

      MYC

      SELP

      VTN

      GDC

      G

      CDH3

      CTNNB1

      EGR3

      GLI2

      IL20

      IVL

      KRT17

      LAMB2

      NOTCH1

      SHH

      WNT1

      PPC

      H

      CDK1

      CYR61

      FABP5

      HGF

      IL36RN

      JAG1

      KRT18

      LAMB3

      PSORS1C1

      SMO

      WNT3A

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK100
      產地美國
      縮寫GQH-KCB
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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