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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類平滑肌細胞生物學qPCR檢測試劑盒

      英文名:Human Smooth Muscle Cell Biology qPCR Array Kit

      貨號:GK097

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Smooth Muscle Cell Biology qPCR Array Kit (GQH-SMC) is designed to facilitate gene expression profiling of 88 key genes involved in human smooth muscle cell (SMC) biology, such as vascular SMC contraction, SMC phenotypic switching, and the extracellular matrix (ECM) synthesis. Genes implicated in selected SMC related disorders are also included in the array. Brief examples of how genes may be grouped according to their functions are shown below:     

      • SMC markers: ACTA2, CDH5, CALD1, HEXIM1, HRH2, MLNR, TAGLN, DES, CSPG4, SMTN, MKL1/2
      • Vascular SMC contraction: ANO1, ADRA1s, NPR1/2, GNAs, MYLKs, ADORA2s, EDNRA, MYH11, MYLs
      • SMC phenotypic switching: MYH11, MEF2B, SRF, ELK1, MYOCD, GATA6, MKL1/2, RHOA, TGFB1, NOTCH1/3, HEY2
      • SMC heterogeneity: ACTA2, TAGLN, CNN2, MYH11, OGN, AQP1, PLN, MGP, SPP1, KDR, SMTN, VCL, DES
      • ECM synthesis: LAMA5, collagens, ELN, OGN, MGP, SPP1, FGA/B/G
      • Genes implicated in
        • systemic hypertension: PTGIS, AGT, ADD1, AGTR1, GNB3, NOS3
        • achalasia: NOS1, AAAS, GMPPA, GUCY1A3, HLA-DQA1/B1
        • bronchospasm/asthma: HNMT, IL13, CCL11, ADRB2, ALOX5, SCGB3A2

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human Smooth Muscle Cell Biology qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      AAAS

      ADRB2

      AVPR1B

      COL3A1

      ELN

      GNA13

      HRH2

      MLNR

      MYOCD

      NPR2

      SCGB3A2

      β-actin

      B

      ACTA2

      AEBP1

      CALCRL

      COL4A1

      FGA

      GNB3

      IL13

      MYH11

      NKX3-1

      OGN

      SMAD3

      GAPDH

      C

      ADD1

      AGT

      CALD1

      CSPG4

      FGB

      GUCY1A3

      KDR

      MYL6

      NOS1

      PLN

      SMTN

      LDHA

      D

      ADORA2A

      AGTR1

      CCL11

      CYP4A11

      FGG

      HEXIM1

      LAMA5

      MYL6B

      NOS3

      PTGIR

      SPP1

      NONO

      E

      ADORA2B

      ALOX5

      CDH5

      CYP4A22

      GATA6

      HEY2

      MEF2B

      MYL9

      NOTCH1

      PTGIS

      SRF

      PPIH

      F

      ADRA1A

      ANO1

      CNN2

      DES

      GMPPA

      HLA-DQA1

      MGP

      MYLK

      NOTCH3

      RBP1

      TAGLN

      GDC

      G

      ADRA1B

      AQP1

      COL1A1

      EDNRA

      GNA11

      HLA-DQB1

      MKL1

      MYLK2

      NOX4

      RBPJ

      TGFB1

      PPC

      H

      ADRA1D

      AVPR1A

      COL1A2

      ELK1

      GNA12

      HNMT

      MKL2

      MYLK3

      NPR1

      RHOA

      VCL

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK097
      產地美國
      縮寫GQH-SMC
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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