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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        您當前所在位置:首 頁 > 產品展示 > 基因表達分析 > qPCR檢測試劑盒 人間充質至上皮細胞轉化qPCR檢測試劑盒

        人間充質至上皮細胞轉化qPCR檢測試劑盒

        英文名:Human Mesenchymal to Epithelial Transition qPCR Array Kit

        貨號:GK095

        價格:$250

        產品說明書下載:

        產品預訂,提交訂單信息之后,我們會第一時間與您取得聯系!

        ScienCell's GeneQuery? Human Mesenchymal To Epithelial Transition qPCR Array Kit (GQH-MET) is designed to facilitate gene expression profiling of 88 key genes involved in reversible mesenchymal to epithelial transition (MET). MET and the reverse process, epithelial to mesenchymal transition (EMT), are involved in embryogenesis, cancer metastasis, and stem cell reprogramming. Brief examples of how genes may be grouped are shown below:     

        • EMT markers: CDH1, CDH2, VIM, FN1, CDH11, SDC1, S100A4, ACTA2, CTNNB1, LEF1, ETS1, FOXC2, GSC, PDGFD
        • EMT-MET transition: PAX2, WT1, BMP7, TWIST1, PRRX1
        • Proliferation and morphogenesis control: AKT1, BMP1, BMP7, EGFR, FGFBP1, IGFBP4, JAG1, PDGFRB, SNAI1, SOX10, VCAN, WNT5A, WNT11, ZEB1
        • Differentiation control: BMP2, COL3A1, ERBB3, F11R, FZD7, GSC, JAG1, KRT14, NODAL, SMAD2, SOX10, WNT5B
        • Transcription factors regulating MET: CTNNB1, ESR1, FOXC2, NOTCH1, SMAD2, SNAI1/2/3, STAT3, TCF3, TCF4, TWIST1, ZEB2
        • Signaling pathways involved in MET
          • Estrogen receptor: CAV2, ESR1, KRT19, TGFB3
          • GPCR: AKT1, FZD7, GNG11, RAC1, RGS2
          • PI3K/AKT: AKT1, AKT2, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PIK3R3, PIK3R5

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

        GeneQuery? Human Mesenchymal To Epithelial Transition qPCR Array Plate Layout*

        (8 controls in Bold and Italic)

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ACTA2

        CAV2

        COL3A1

        F11R

        IGFBP4

        MUC1

        PIK3CG

        RGS2

        SNAI3

        TGFB2

        VCAN

        β-actin

        B

        AKT1

        CDH1

        COL5A2

        FGFBP1

        ILK

        NODAL

        PIK3R1

        RPS27A

        SOX10

        TGFB3

        VIM

        GAPDH

        C

        AKT2

        CDH11

        CTNNB1

        FN1

        JAG1

        NOTCH1

        PIK3R3

        S100A4

        SPINT2

        TGFBR1

        WNT11

        LDHA

        D

        ARHGEF18

        CDH2

        DSP

        FOXC2

        KRT14

        PAX2

        PIK3R5

        SDC1

        STAT3

        TGFBR2

        WNT5A

        NONO

        E

        AXL

        CDKN2A

        EGFR

        FZD7

        KRT19

        PDGFD

        PRKCZ

        SMAD2

        STK11

        TIMP1

        WNT5B

        PPIH

        F

        BMP1

        CDKN2B

        ERBB3

        GNG11

        LEF1

        PDGFRB

        PRRX1

        SMURF1

        TCF3

        TRIM28

        WT1

        GDC

        G

        BMP2

        CLDN4

        ESR1

        GSC

        MMP2

        PIK3CA

        RAB25

        SNAI1

        TCF4

        TWIST1

        ZEB1

        PPC

        H

        BMP7

        CLDN7

        ETS1

        HNRNPAB

        MST1R

        PIK3CD

        RAC1

        SNAI2

        TGFB1

        UBA52

        ZEB2

        NTC

        * gene selection may be updated based on new research and development

        貨號GK095
        產地美國
        縮寫GQH-MET
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看

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