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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      Human Renal Mesangial Cell Biology qPCR Array Kit

      英文名:Human Renal Mesangial Cell Biology qPCR Array Kit

      貨號:GK093

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Renal Mesangial Cell Biology qPCR Array Kit (GQH-RMB) is designed to facilitate gene expression profiling of 88 key genes involved in essential mesangial cell biology and functions involved in glomerular physiology and pathophysiology. Brief examples of how included genes may be grouped according to their functions are shown below:     

      • Renal mesangial cell markers: ACTA1, FN1, PDGFRB, TNS1, ITGA8
      • Mesangial matrix assembly: SERPINB7, Collagen type 4/5, Laminin α/β/γ, SDC2, HSPG2, CSPG5, NID1, ITGA8
      • Growth factor modulation: IGF1, PTN, MFGE8, GDF10, CTGF, NGF, PSEN2, SH3KBP1, ADAM12, AFAP1L2, SHC3
      • Vasculogenesis: VCAM1, ANGPT2, HOPX, MYH11, NRP2, HDAC9, GJA5, ADAMTS1, FHL2, PLN, CTGF
      • Endocytosis: CLTA, CLTB, CLTC, AP2A1, AP2A2, AP2B1, AP2M1, AP2S1, SMAD2, SMAD3, ZFYVE9, TGFB receptors
      • Transmembrane transporter activity: KCNMB1, GJA5, CACNA2D1, ABCC9, P2RX1, KCNRG, TRPCs, KCNQ5, PIEZO2
      • Genes implicated in
        • Mesangial proliferative glomerulonephritis: SERPINB7, IL6, CD79A, CCL2, FGF2, EDN1, CCR5, GAS6, AXL
        • Diffuse mesangial sclerosis: ACTN4, TRPC6, INF2, WT1, PLCE1, PAX2, ARHGDIA, LAMB2, LAMC1

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human Renal Mesangial Cell Biology qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ABCC9

      AP2A1

      CCL2

      COL5A1

      FHL2

      IGF1

      LAMA2

      MFGE8

      PDGFRB

      SFRP2

      TGFBR3

      β-actin

      B

      ACTA1

      AP2A2

      CCR5

      COL5A2

      FN1

      IL6

      LAMA3

      MYH11

      PIEZO2

      SH3KBP1

      TNS1

      GAPDH

      C

      ACTN4

      AP2B1

      CD79A

      COL5A3

      GAS6

      INF2

      LAMA4

      NGF

      PLCE1

      SHC3

      TRPC1

      LDHA

      D

      ADAM12

      AP2M1

      CLTA

      CSPG5

      GDF10

      ITGA8

      LAMA5

      NID1

      PLN

      SMAD1

      TRPC4

      NONO

      E

      ADAMTS1

      AP2S1

      CLTB

      CTGF

      GJA5

      KCNMB1

      LAMB1

      NID2

      PSEN2

      SMAD2

      TRPC6

      PPIH

      F

      AFAP1L2

      ARHGDIA

      CLTC

      EDN1

      HDAC9

      KCNQ5

      LAMB2

      NRP2

      PTN

      SMAD3

      VCAM1

      GDC

      G

      AGTR1

      AXL

      COL4A1

      ETS1

      HOPX

      KCNRG

      LAMC1

      P2RX1

      SDC2

      TGFBR1

      WT1

      PPC

      H

      ANGPT2

      CACNA2D1

      COL4A2

      FGF2

      HSPG2

      LAMA1

      MECOM

      PAX2

      SERPINB7

      TGFBR2

      ZFYVE9

      NTC

      * gene selection may be updated based on new research and development

      貨號GK093
      產地美國
      縮寫GQH-RMB
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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