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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類Ephrin信號qPCR檢測試劑盒

      英文名:Human Ephrin Signaling qPCR Array Kit

      貨號:GK089

      價格:$250

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      ScienCell's GeneQuery? Human Ephrin Signaling qPCR Array Kit (GQH-EPH) profiles 88 key genes involved in ephrin signaling and signal transduction.  Ephrin signaling is involved in such developmental processes as axonal guidance, angiogenesis, cell migration, embryonic development, and segmentation.  Ephrin ligands and receptors are subdivided into two classes based on their structure and binding affinities: ephrin-A and ephrin-B.  A more unique aspect of ephrin signaling is that it can occur bidirectionally expressing as both a “forward signal” and a “reverse signal.”  Brief examples of how included genes may be grouped according to their function are shown below:

      • Signaling receptors: EPHA2, EPHA4, EPHB1, EPHB2, EPHB4
      • Ligands: EFNA1, EFNA2, EFNA5, EFNB1, EFNB2
      • Cytoskeletal dynamics: ACTB, ACTG1, ACTN1, CFL1, DNM1
      • Vesicle Trafficking: AP2B1, AP2S1, APH1A, CAV1, CLTA
      • Hemostasis: FN1, FYN, ITGAVM ITGB1, LYN

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Ephrin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACTB

      AP2S1

      DNM1

      EFNB3

      EPHB2

      GRIA1

      LIMK1

      MYL6

      PIK3CG

      RAP1A

      TF

      β-actin

      B

      ACTG1

      APH1A

      EFNA1

      EPHA1

      EPHB3

      GRIN1

      LYN

      MYL9

      PIK3R1

      RASA1

      TIAM1

      GAPDH

      C

      ACTN1

      CAV1

      EFNA2

      EPHA2

      EPHB4

      GRIN2B

      MAP2K1

      NCK1

      PSEN2

      RGS3

      TLN1

      LDHA

      D

      ADAM10

      CDC42

      EFNA3

      EPHA3

      FN1

      HRAS

      MAPK1

      NCK2

      PSENEN

      RHOA

      VAV2

      NONO

      E

      AP2A1

      CFL1

      EFNA4

      EPHA4

      FYN

      ITGA5

      MMP2

      NCSTN

      PTK2

      ROCK1

      VAV3

      PPIH

      F

      AP2A2

      CLTA

      EFNA5

      EPHA5

      GNAI1

      ITGAV

      MMP9

      PAK1

      PXN

      SDC2

      VCL

      GDC

      G

      AP2B1

      CLTB

      EFNB1

      EPHA7

      GRB2

      ITGB1

      MYH10

      PAK2

      RAC1

      SRC

      VEGFA

      PPC

      H

      AP2M1

      CLTC

      EFNB2

      EPHB1

      GRB7

      KDR

      MYH9

      PIK3CA

      RAF1

      SYNJ1

      WASL

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK089
      產地美國
      縮寫GQH-EPH
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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