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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        人類Ephrin信號qPCR檢測試劑盒

        英文名:Human Ephrin Signaling qPCR Array Kit

        貨號:GK089

        價格:$250

        產品說明書下載:

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        ScienCell's GeneQuery? Human Ephrin Signaling qPCR Array Kit (GQH-EPH) profiles 88 key genes involved in ephrin signaling and signal transduction.  Ephrin signaling is involved in such developmental processes as axonal guidance, angiogenesis, cell migration, embryonic development, and segmentation.  Ephrin ligands and receptors are subdivided into two classes based on their structure and binding affinities: ephrin-A and ephrin-B.  A more unique aspect of ephrin signaling is that it can occur bidirectionally expressing as both a “forward signal” and a “reverse signal.”  Brief examples of how included genes may be grouped according to their function are shown below:

        • Signaling receptors: EPHA2, EPHA4, EPHB1, EPHB2, EPHB4
        • Ligands: EFNA1, EFNA2, EFNA5, EFNB1, EFNB2
        • Cytoskeletal dynamics: ACTB, ACTG1, ACTN1, CFL1, DNM1
        • Vesicle Trafficking: AP2B1, AP2S1, APH1A, CAV1, CLTA
        • Hemostasis: FN1, FYN, ITGAVM ITGB1, LYN

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

        GeneQuery? Human Ephrin Signaling qPCR Array Plate Layout* (8 controls in Bold and Italic)

         

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ACTB

        AP2S1

        DNM1

        EFNB3

        EPHB2

        GRIA1

        LIMK1

        MYL6

        PIK3CG

        RAP1A

        TF

        β-actin

        B

        ACTG1

        APH1A

        EFNA1

        EPHA1

        EPHB3

        GRIN1

        LYN

        MYL9

        PIK3R1

        RASA1

        TIAM1

        GAPDH

        C

        ACTN1

        CAV1

        EFNA2

        EPHA2

        EPHB4

        GRIN2B

        MAP2K1

        NCK1

        PSEN2

        RGS3

        TLN1

        LDHA

        D

        ADAM10

        CDC42

        EFNA3

        EPHA3

        FN1

        HRAS

        MAPK1

        NCK2

        PSENEN

        RHOA

        VAV2

        NONO

        E

        AP2A1

        CFL1

        EFNA4

        EPHA4

        FYN

        ITGA5

        MMP2

        NCSTN

        PTK2

        ROCK1

        VAV3

        PPIH

        F

        AP2A2

        CLTA

        EFNA5

        EPHA5

        GNAI1

        ITGAV

        MMP9

        PAK1

        PXN

        SDC2

        VCL

        GDC

        G

        AP2B1

        CLTB

        EFNB1

        EPHA7

        GRB2

        ITGB1

        MYH10

        PAK2

        RAC1

        SRC

        VEGFA

        PPC

        H

        AP2M1

        CLTC

        EFNB2

        EPHB1

        GRB7

        KDR

        MYH9

        PIK3CA

        RAF1

        SYNJ1

        WASL

        NTC

         

         * gene selection may be updated based on new research and development

        貨號GK089
        產地美國
        縮寫GQH-EPH
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看
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