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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人VEGF信號通路qPCR檢測試劑盒

      英文名:Human VEGF Signaling Pathway qPCR Array Kit

      貨號:GK088

      價格:$250

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      ScienCell's GeneQuery? Human Signaling Pathway qPCR Array Kit (GQH-VEG) profiles 88 key genes involved in VEGF and VEGF-related signaling.  VEGF is a growth factor known for stimulating angiogenesis and vasculogenesis.  As such, it is integral to proper blood vasculature formation during development, maintenance, and wound healing.  Aberrant VEGF signaling is associated with ischemia, diabetic retinopathy, macular degeneration, and solid tumor progression.  Brief examples of how included genes may be grouped according to their function are shown below:

      • Signaling receptors: FLT1, FLT4, KDR, KIT, FGFR1
      • Growth factor ligands: VEGFA, VEGFB, VEGFC, FGF2, PGF
      • Cytoskeletal response: ACTA1, ACTA2, ACTB, ACTG1, ACTG2
      • Adhesion regulation: ITGAV, ITGB1, ITGB3, ITGB5, PTK2
      • Downstream transcription factors: ATF2, FOS, JUN, KRAS, NRAS

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human VEGF Signaling Pathway qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACTA1

      ATF2

      FGF2

      FN1

      ITGB3

      L1CAM

      MAPK3

      NRP2

      PRKCA

      PRKCZ

      SOS1

      β-actin

      B

      ACTA2

      BAD

      FGFR1

      FOS

      ITGB5

      MAP2K1

      MAPK8

      PGF

      PRKCB

      PTEN

      SOS2

      GAPDH

      C

      ACTB

      BCAR1

      FGFR2

      GNA12

      ITPR1

      MAP2K2

      MAPK9

      PIK3C2A

      PRKCD

      PTK2

      SRF

      LDHA

      D

      ACTG1

      CASP9

      FGFR3

      GNA13

      ITPR2

      MAP2K7

      MEF2A

      PIK3R2

      PRKCE

      PXN

      TIMP3

      NONO

      E

      ACTG2

      CRK

      FGFR4

      GRB2

      JUN

      MAPK1

      MYL9

      PIK3R4

      PRKCG

      RAF1

      VEGFA

      PPIH

      F

      AKT1

      CSK

      FGFRL1

      HRAS

      KDR

      MAPK10

      NOS3

      PLA2G4A

      PRKCH

      ROCK1

      VEGFB

      GDC

      G

      AKT2

      EGF

      FLT1

      ITGAV

      KIT

      MAPK12

      NRAS

      PLCG1

      PRKCI

      RRAS

      VEGFC

      PPC

      H

      AKT3

      ELK1

      FLT4

      ITGB1

      KRAS

      MAPK13

      NRP1

      PLCG2

      PRKCQ

      SHC1

      VEGFD

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK088
      產地美國
      縮寫GQH-VEG
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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