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      人體軸突導向qPCR檢測試劑盒

      英文名:Human Axon Guidance qPCR Array kit

      貨號:GK084

      價格:$250

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      ScienCell's GeneQuery? Human Axon Guidance qPCR Array (GQH-AXN) profiles 88 key genes involved in neural pathfinding.  During neural development, neurons send projections otherwise known as axons to reach targets and transmit electric signals.  Axons follow specific paths and the underlying pathfinding mechanism is an area of growing research.  Below are brief examples of how included genes may be grouped according to their signaling pathways or protein classes:

      • Ephrins: EFNA1, EFNA5, EFNB3, EPHA1, EPHB1
      • Semaphorins: SEMA3A, SEMA4D, SEMA5A, SEMA6B, SEMA7A
      • WNT signaling: WNT4, WNT5A, SHH, SMO, FZD3
      • Netrin signaling: NTN1, NTN4, NTNG1, NTNG2, DCC, UNC5A
      • Cell adhesion molecules: CAMK2A, CAMK2B, CAMK2D, CAMK2G

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Axon Guidance qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ABL1

      CAMK2B

      EFNA1

      EPHA1

      EPHB3

      NRP1

      RAC1

      SEMA3D

      SEMA4F

      SLIT2

      TRPC6

      β-actin

      B

      ABLIM3

      CAMK2D

      EFNA2

      EPHA2

      EPHB4

      NRP2

      RAF1

      SEMA3E

      SEMA4G

      SLIT3

      UNC5A

      GAPDH

      C

      ARHGEF12

      CAMK2G

      EFNA3

      EPHA3

      F2RL2

      NTN1

      ROBO1

      SEMA3F

      SEMA5A

      SMO

      UNC5B

      LDHA

      D

      BMP7

      CFL1

      EFNA4

      EPHA4

      FES

      NTN4

      ROBO2

      SEMA3G

      SEMA6B

      TRPC1

      UNC5C

      NONO

      E

      BMPR1B

      CFL2

      EFNA5

      EPHA5

      FYN

      NTNG1

      ROCK1

      SEMA4A

      SEMA6D

      TRPC1

      UNC5D

      PPIH

      F

      BMPR2

      CXCR4

      EFNB1

      EPHA7

      FZD3

      NTNG2

      RYK

      SEMA4B

      SEMA7A

      TRPC3

      WNT4

      GDC

      G

      BOC

      DCC

      EFNB2

      EPHB1

      NCK1

      PAK2

      SEMA3A

      SEMA4C

      SHH

      TRPC4

      WNT5A

      PPC

      H

      CAMK2A

      DPYSL2

      EFNB3

      EPHB2

      NFATC2

      PAK4

      SEMA3C

      SEMA4D

      SLIT1

      TRPC5

      WNT5B

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK084
      產地美國
      縮寫GQH-AXN
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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