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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人成骨分化qPCR檢測試劑盒

      英文名:GeneQuery? Human Osteogenic Differentiation qPCR Array Kit

      貨號:GK080

      價格:$250

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      ScienCell's GeneQuery? Human Osteogenic Differentiation qPCR Array (GQH-OST) facilitates gene expression profiling of 88 key genes involved in the differentiation of mesenchymal stem cells into osteocytes, osteoblasts, and osteoclasts.  Osteocytes are most commonly found in mature bone tissue and capable of signal transmission over long distances.  Osteoblasts and osteoclasts are bone cells that synthesize and break down bone, respectively.  Brief examples of how included genes may be grouped according to their function are shown below:

      • Osteocyte marker: BGN, FGF23, PDPN, HYOU1, SOST
      • Osteoblast marker: GNL3, CD44, OMD, MME, SCUBE3
      • Osteoclast marker: CA2, CALCR, CTSK, MMP9, TNFRSF11A
      • RUNX2 suppressor: GLI3, HES1, STAT1, TWIST1, HAND2
      • BMP signaling: BMP2, BMP4, SMAD4, SMAD5, SMAD9

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Osteogenic Differentiation qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ALCAM

      CA2

      CSNK2A1

      FGF2

      FZD9

      HYOU1

      MAF

      NKX3-2

      RBPJ

      SMO

      TCF7

      β-actin

      B

      ALPL

      CALCR

      CTNNB1

      FGF23

      GLI2

      IBSP

      MCAM

      NOTCH1

      RUNX2

      SOST

      TGFB1

      GAPDH

      C

      ATF4

      CAPG

      CTSK

      FGFR1

      GLI3

      IGF1R

      MEPE

      NOTCH2

      SATB2

      SOX9

      TNFRSF11A

      LDHA

      D

      BCAP31

      CD44

      DCN

      FGFR2

      GNL3

      IGFBP3

      MME

      OMD

      SCUBE3

      SP7

      TNFSF11

      NONO

      E

      BGN

      CD59

      DMP1

      FGFR3

      HAND2

      ITGB1

      MMP14

      PCOLCE

      SMAD1

      SPARC

      TPO

      PPIH

      F

      BMP2

      COL1A1

      DSTN

      FN1

      HES1

      LEF1

      MMP9

      PDPN

      SMAD4

      SPP1

      TWIST1

      GDC

      G

      BMP4

      COL1A2

      ENG

      FOSB

      HEY1

      LRP5

      MSX2

      PHEX

      SMAD5

      STAT1

      TXLNG

      PPC

      H

      BMPR1A

      COL2A1

      FGF18

      FOSL1

      HIVEP3

      LRP6

      NFATC1

      PRKCD

      SMAD9

      TAZ

      WNT5A

      NTC

       * gene selection may be updated based on new research and development

      貨號GK080
      產地美國
      縮寫GQH-OST
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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