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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        人成骨分化qPCR檢測試劑盒

        英文名:GeneQuery? Human Osteogenic Differentiation qPCR Array Kit

        貨號:GK080

        價格:$250

        產品說明書下載:

        產品預訂,提交訂單信息之后,我們會第一時間與您取得聯系!

        ScienCell's GeneQuery? Human Osteogenic Differentiation qPCR Array (GQH-OST) facilitates gene expression profiling of 88 key genes involved in the differentiation of mesenchymal stem cells into osteocytes, osteoblasts, and osteoclasts.  Osteocytes are most commonly found in mature bone tissue and capable of signal transmission over long distances.  Osteoblasts and osteoclasts are bone cells that synthesize and break down bone, respectively.  Brief examples of how included genes may be grouped according to their function are shown below:

        • Osteocyte marker: BGN, FGF23, PDPN, HYOU1, SOST
        • Osteoblast marker: GNL3, CD44, OMD, MME, SCUBE3
        • Osteoclast marker: CA2, CALCR, CTSK, MMP9, TNFRSF11A
        • RUNX2 suppressor: GLI3, HES1, STAT1, TWIST1, HAND2
        • BMP signaling: BMP2, BMP4, SMAD4, SMAD5, SMAD9

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

        GeneQuery? Human Osteogenic Differentiation qPCR Array Plate Layout* (8 controls in Bold and Italic)

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ALCAM

        CA2

        CSNK2A1

        FGF2

        FZD9

        HYOU1

        MAF

        NKX3-2

        RBPJ

        SMO

        TCF7

        β-actin

        B

        ALPL

        CALCR

        CTNNB1

        FGF23

        GLI2

        IBSP

        MCAM

        NOTCH1

        RUNX2

        SOST

        TGFB1

        GAPDH

        C

        ATF4

        CAPG

        CTSK

        FGFR1

        GLI3

        IGF1R

        MEPE

        NOTCH2

        SATB2

        SOX9

        TNFRSF11A

        LDHA

        D

        BCAP31

        CD44

        DCN

        FGFR2

        GNL3

        IGFBP3

        MME

        OMD

        SCUBE3

        SP7

        TNFSF11

        NONO

        E

        BGN

        CD59

        DMP1

        FGFR3

        HAND2

        ITGB1

        MMP14

        PCOLCE

        SMAD1

        SPARC

        TPO

        PPIH

        F

        BMP2

        COL1A1

        DSTN

        FN1

        HES1

        LEF1

        MMP9

        PDPN

        SMAD4

        SPP1

        TWIST1

        GDC

        G

        BMP4

        COL1A2

        ENG

        FOSB

        HEY1

        LRP5

        MSX2

        PHEX

        SMAD5

        STAT1

        TXLNG

        PPC

        H

        BMPR1A

        COL2A1

        FGF18

        FOSL1

        HIVEP3

        LRP6

        NFATC1

        PRKCD

        SMAD9

        TAZ

        WNT5A

        NTC

         * gene selection may be updated based on new research and development

        貨號GK080
        產地美國
        縮寫GQH-OST
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看
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