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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類軟骨細胞生物學qPCR檢測試劑盒

      英文名:Human Chondrocyte Biology qPCR Array Kit

      貨號:GK079

      價格:$250

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      ScienCell's GeneQuery? Human Chondrocyte Biology qPCR Array Kit (GQH-CHN) facilitates gene expression profiling of 88 key genes in chondrocyte function.  Chondrocytes are embedded in the cartilage matrix and are responsible for producing and maintaining it.  Chondrocytes originate in the mesenchyme and are hypertrophic when terminally differentiated during ossification.  Brief examples of how included genes may be grouped according to their function in chondrocyte biology are shown below:

      • Chondrogenesis: COL2A1, ACAN, SOX9, MIA, PTCH1
      • ECM interaction: RHOA, ITGAV, CD44, PTK2, ITGB1
      • Mechanotransduction: NFATC4, TRPV4, CREB1, MAPK1, PRKCE
      • Remodeling and repair: BMP2, HIF1A, MMP9, WNT9A, ICAM1

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Chondrocyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACAN

      CACNA1C

      COL2A1

      FGFR3

      IGF1R

      ITGA6

      MAPK3

      NFATC4

      PTGER4

      RUNX2

      SOX9

      β-actin

      B

      ALPL

      CACNA1D

      COL4A1

      FLNA

      IHH

      ITGA7

      MATN1

      NKX3-2

      PTH1R

      SCNN1A

      SP7

      GAPDH

      C

      ANXA5

      CD151

      COL4A2

      FLNB

      ILK

      ITGAV

      MATN3

      OTOR

      PTHLH

      SCNN1B

      SPARC

      LDHA

      D

      ANXA6

      CD44

      CREB1

      FOXC1

      ITGA1

      ITGB1

      MATN4

      PRG4

      PTK2

      SCNN1G

      TGFB3

      NONO

      E

      AQP1

      CD47

      CRTAC1

      FOXC2

      ITGA2

      ITGB5

      MIA

      PRKCA

      PXN

      SMAD2

      TNC

      PPIH

      F

      BMP2

      CHAD

      CTSB

      HIF1A

      ITGA3

      KCNA4

      MMP13

      PRKCB

      RAC1

      SMAD3

      TRPV4

      GDC

      G

      BMP4

      CILP

      DDR2

      IBSP

      ITGA4

      KCNA6

      MMP9

      PRKCE

      RARG

      SOX5

      VEGFA

      PPC

      H

      CACNA1A

      COL11A2

      EPYC

      ICAM1

      ITGA5

      MAPK1

      NCAM1

      PTCH1

      RHOA

      SOX6

      WNT9A

      NTC

       * gene selection may be updated based on new research and development

      貨號GK079
      產地美國
      縮寫GQH-CHN
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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