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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人角質細胞生物學qPCR檢測

      英文名:Human Keratocyte Biology qPCR Array

      貨號:GK074

      價格:$250

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      ScienCell's GeneQuery? Human Keratocyte Biology qPCR Array (GQH-KER) profiles 88 key genes involved in corneal keratocyte function.  Keratocytes are specialized fibroblasts that play roles in wound healing, maintaining corneal transparency, and corneal component synthesis.  Keratocyte pathologies include keratoconus and limbal stem cell deficiency.  Below are brief examples of how included genes may be grouped according to their function in keratocyte biology:

      • Markers: MUC1, CD34, KERA, KRT19, LUM
      • Wound Healing: MMP12, MRC1, NFKB1, COL3A1, CCL2
      • Cell Cycling: MAPK1, MYCN, PTN, ETS1, CAPN9
      • Oxidative Stress Response: RRAS, NOS2, NQO1, MAF, HMOX1
      • Keratoconus Development: IGFBP3, MMP1, SSTR1, CRX, TNFAIP6

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Keratocyte Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACTA1

      CCL2

      COL1A1

      CRYAA

      F13A1

      GSTK1

      KERA

      MAPK3

      NCAM1

      PTN

      TGFBR1

      β-actin

      B

      ACTA2

      CCL7

      COL1A2

      CRYAB

      FASLG

      HMOX1

      KRT1

      MMP1

      NFKB1

      RRAS

      TGFBR2

      GAPDH

      C

      ALDH1A1

      CD34

      COL3A1

      CRYBB1

      FGF2

      ICAM1

      KRT12

      MMP12

      NFKB2

      SDC1

      TIMP1

      LDHA

      D

      ATF1

      CD68

      COL5A1

      CRYGD

      FGFR1

      IGF1R

      KRT19

      MMP2

      NOS2

      SOD2

      TIMP2

      NONO

      E

      BGN

      CDK1

      COL5A2

      CXADR

      FGFR2

      IGFBP3

      LCN2

      MMP3

      NQO1

      SPARC

      TIMP3

      PPIH

      F

      BMP4

      CFL1

      COL6A2

      ECM1

      FN1

      IGFBP5

      LUM

      MRC1

      PCNA

      SSTR1

      TKT

      GDC

      G

      C12orf57

      CHST6

      COL7A1

      EPHX1

      FOS

      IL1A

      MAF

      MUC1

      PDGFRB

      TC2N

      TNFAIP6

      PPC

      H

      CAPN9

      CLU

      CRX

      ETS1

      FOSL1

      IL6

      MAPK1

      MYCN

      PTGDS

      TGFB1

      VCL

      NTC

        * gene selection may be updated based on new research and development

      貨號GK074
      產地美國
      縮寫GQH-KER
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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