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      人類適應性免疫反應qPCR檢測試劑盒

      英文名:Human Adaptive Immune Response qPCR Array Kit

      貨號:GK072

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Adaptive Immune Response qPCR Array (GQH-AIR) facilitates gene expression profiling of 88 key genes involved in adaptive immunity or acquired immunity.  Vertebrates have two main immune systems: innate and adaptive.  While the innate immune system is responsible for host defense against pathogens in a general way, the adaptive immune system is responsible for defense against particular pathogens, thereby conferring immunological memory.  Brief examples of how included genes may be grouped according to their function in the adaptive immune response are shown below:

      • Antigen presentation: CANX, HLA-A, HLA-DPB1, PSMB8, TAP2
      • Th1 markers: IL12RB1, CCR5, CD94, CXCR3. IL18R
      • Th2 markers: IL13, IL1R1, IL4, CCR8, CSF2
      • Th17 markers: CCR6, IL17A, IL6, RORC, IRF4
      • Treg markers: FOXP3, CD4, IL10, IL2RA, CTLA4

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Adaptive Immune Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ABCC1

      CCR8

      CD40LG

      FOXP3

      HLA-DQA1

      IL12RB1

      IL1R1

      IRF4

      NOTCH3

      PTPRC

      STAT6

      β-actin

      B

      ARG1

      CD19

      CD80

      GATA3

      HLA-DQB1

      IL12RB2

      IL1R2

      ITGAM

      PDIA3

      RAG1

      TAP2

      GAPDH

      C

      CANX

      CD27

      CD86

      HAVCR2

      HLA-DRA

      IL13

      IL2

      JAK3

      PSMB8

      RAG2

      TBX21

      LDHA

      D

      CCL5

      CD28

      CSF2

      HLA-A

      HLA-DRB1

      IL17A

      IL2RA

      KLRD1

      PSMB9

      RORA

      TNF

      NONO

      E

      CCR1

      CD3D

      CTLA4

      HLA-B

      HLA-DRB3

      IL17B

      IL4

      LGALS1

      PSME1

      RORC

      TNFRSF4

      PPIH

      F

      CCR4

      CD3E

      CXCR3

      HLA-C

      IFNG

      IL17C

      IL5

      LTA

      PSME2

      STAT1

      TNFSF11

      GDC

      G

      CCR5

      CD3G

      CXCR4

      HLA-DPA1

      IL10

      IL18

      IL6

      MS4A1

      PSME3

      STAT3

      TRAT1

      PPC

      H

      CCR6

      CD4

      CXCR6

      HLA-DPB1

      IL12A

      IL18R1

      IL9

      NFKB1

      PTGDR2

      STAT4

      ZAP70

      NTC

       * gene selection may be updated based on new research and development

      貨號GK072
      產地美國
      縮寫GQH-AIR
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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