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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類DNA損傷感應qPCR檢測試劑盒

      英文名:Human DNA Damage Sensing qPCR Array Kit

      貨號:GK061

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human DNA Damage Sensing qPCR Array Kit (GQH-DDS) is designed to facilitate gene expression profiling of 40 key genes involved in DNA damage sensing. DNA damages, such as mutations, breaks, cross-links, deletions/insertions and translocations, are constantly generated due to internal and external stresses. Appropriate DNA damage responses must be implemented to maintain genome stability. Brief examples of how included genes may be grouped are shown below:

      • DNA damage sensors: ATM, ATR, PRKDC, PARP1
      • Cell cycle regulation: CHEK1, CHEK2, CCNB1, CCNB2, CCNB3, CCND1, CCND2, CCND3, CCNE2, CDK2, CDK4, CDK6
      • ATM signaling pathway: ABL1, BRCA1, CDC25A, CDKN1A, CHEK1, CHEK2, GADD45A, RAD51, RBBP8, TP53
      • ATR signaling pathway: CDC25A, CDC25C, CDK2, CHEK1, CHEK2, HUS1, MDM2, RAD1, RAD17, RAD9A, TP53
      • Apoptosis: ABL1, ATM, BAX, BRCA1, CASP3, CASP9, CDKN1A, CHEK2, PRKDC, RAD91, TP53

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human DNA Damage Sensing qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ABL1

      CCNB1

      CDC25C

      FAS

      PRKDC

      β-actin

      ABL1

      CCNB1

      CDC25C

      FAS

      PRKDC

      β-actin

      B

      ATM

      CCNB2

      CDK2

      GADD45A

      RAD1

      GAPDH

      ATM

      CCNB2

      CDK2

      GADD45A

      RAD1

      GAPDH

      C

      ATR

      CCNB3

      CDK4

      GADD45B

      RAD17

      LDHA

      ATR

      CCNB3

      CDK4

      GADD45B

      RAD17

      LDHA

      D

      BAX

      CCND1

      CDK6

      HUS1

      RAD51

      NONO

      BAX

      CCND1

      CDK6

      HUS1

      RAD51

      NONO

      E

      BID

      CCND2

      CDKN1A

      MDM2

      RAD9A

      PPIH

      BID

      CCND2

      CDKN1A

      MDM2

      RAD9A

      PPIH

      F

      BRCA1

      CCND3

      CDKN1B

      MYC

      RB1

      GDC

      BRCA1

      CCND3

      CDKN1B

      MYC

      RB1

      GDC

      G

      CASP3

      CCNE2

      CHEK1

      NBN

      RBBP8

      PPC

      CASP3

      CCNE2

      CHEK1

      NBN

      RBBP8

      PPC

      H

      CASP9

      CDC25A

      CHEK2

      PARP1

      TP53

      NTC

      CASP9

      CDC25A

      CHEK2

      PARP1

      TP53

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK061
      產地美國
      縮寫GQH-DDS
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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