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      人類晝夜節律qPCR檢測試劑盒

      英文名:Human Circadian Rhythm qPCR Array Kit

      貨號:GK060

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Circadian Rhythm qPCR Array Kit (GQH-CIR) profiles 88 key genes involved in the regulating changes that occur on a roughly 24-hr cycle.  Circadian rhythms are primarily responses to changes in light and are found in most living organisms.  Not to be confused with biological clocks, circadian rhythms can influence sleep patterns, hormone release, body temperature, and other homeostatic functions.  Below are brief examples of how included genes may be grouped according to their function:

      • Clock regulation: ARNTL, BHLHE40, CLOCK, PER1, RORA
      • Casein kinases: CSNK1A1, CSNK1D, CSNK1E, CSNK2A1
      • CREB pathway: CAMK2A, HTR7, MAPK1, PRKACB, AANAT
      • Transcription factors: SMAD4, IRF1, NFIL3, RORC, SP1,

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Circadian Rhythm qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      AANAT

      BHLHE41

      CREB3

      CSNK2A1

      HDAC1

      KLF9

      MYOD1

      NR2F6

      PRKAB1

      PRKCA

      RORC

      β-actin

      B

      ADORA1

      BTRC

      CREBBP

      CUL1

      HDAC2

      KMT2A

      NCOA2

      PER1

      PRKAB2

      PRKCB

      SIN3A

      GAPDH

      C

      ADORA2A

      CAMK2A

      CRX

      DBP

      HEBP1

      LGR4

      NFIL3

      PER2

      PRKACB

      PROK2

      SKP1

      LDHA

      D

      AHR

      CAMK2B

      CRY1

      EGR1

      HTR7

      MAPK1

      NGFR

      PER3

      PRKACG

      PROX1

      SMAD4

      NONO

      E

      ARNTL

      CDK5R1

      CRY2

      EGR3

      ID1

      MAPK14

      NOCT

      PPARA

      PRKAG2

      RBX1

      SP1

      PPIH

      F

      ARNTL2

      CHEK1

      CSNK1A1

      EPO

      ID2

      MAPK3

      NONO

      PPARGC1A

      PRKAG3

      ROCK2

      SREBF1

      GDC

      G

      ATR

      CLOCK

      CSNK1D

      FBXL3

      IRF1

      MMAB

      NPAS2

      PRKAA1

      PRKAR1A

      RORA

      STAT5A

      PPC

      H

      BHLHE40

      CREB1

      CSNK1E

      FBXW11

      KLF10

      MTNR1B

      NR1D2

      PRKAA2

      PRKAR2A

      RORB

      UBE3A

      NTC 


       * gene selection may be updated based on new research and development

      貨號GK060
      產地美國
      縮寫GQH-CIR
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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