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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人體皮膚傷口愈合qPCR檢測套件

      英文名:Human Skin Wound Healing qPCR Array Kit

      貨號:GK041

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Skin Wound Healing qPCR Array Kit (GQH-SWH) is designed to facilitate gene expression profiling of 88 key genes involved in the wound repair process. Wound healing is a complex process that includes many biological events, such as phagocytosis, angiogenesis, neocollagenesis, epithelialization, and collagen remodeling. It can be generally divided into 3 stages: 1) hemostasis and coagulation, 2) inflammation, and 3) proliferation and tissue remodeling. Brief examples of how included genes may be grouped according to their functions are shown below:     

      • Wound healing stages
        • Hemostasis and coagulation: IL1A, IL1B, IL6, TNF, FGF2, IGF1, TGFs, HIF1A
        • Inflammation: CXCL8, VEGFs, ELANE, CTSG, PRTN3, PLAU, TGFs, PDGFs, TNF, IFNG
        • Proliferation and tissue remodeling:  EGF, FGF7, IGF1, IFNG, CSK, RAC1, RHOA, CDC42
      • Extracellular Matrix components: COL1A2, COL3A1, COL4A1, COL14A1
      • Cellular adhesion: CDH1, ITGAs, ITGBs, MMPs, F3, FGA, TIMP1, ACTA2, ACTC1, TAGLN
      • Cytokines and chemokines: CCL2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL4, IL6
      • Growth factors: CSF2, CSF3, EGF, CTGF, FGFs, PDGFs, TGFs

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

      GeneQuery? Human Skin Wound Healing qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACTA2

      COL1A2

      CSK

      CXCL8

      FGF2

      IL10

      ITGB1

      MMP7

      PDGFRB

      RAC1

      TGFBR3

      β-actin

      B

      ACTC1

      COL3A1

      CTGF

      EGF

      FGF7

      IL1A

      ITGB3

      MMP9

      PLAT

      RHOA

      TIMP1

      GAPDH

      C

      ANGPT1

      COL4A1

      CTNNB1

      EGFR

      FIGF

      IL1B

      ITGB5

      NGF

      PLAU

      SERPINE1

      TNF

      LDHA

      D

      CCL2

      COL4A3

      CTSG

      ELANE

      HBEGF

      IL4

      ITGB6

      PDGFA

      PLAUR

      STAT3

      VEGFA

      NONO

      E

      CD40LG

      COL5A1

      CTSK

      F13A1

      HGF

      IL6

      MAPK1

      PDGFB

      PLG

      TAGLN

      VEGFB

      PPIH

      F

      CDC42

      COL5A3

      CTSV

      F3

      HIF1A

      IL6ST

      MAPK3

      PDGFC

      PRTN3

      TGFA

      VEGFC

      GDC

      G

      CDH1

      CSF2

      CXCL1

      FGA

      IFNG

      ITGA1

      MMP1

      PDGFD

      PTEN

      TGFB1

      WISP1

      PPC

      H

      COL14A1

      CSF3

      CXCL2

      FGF10

      IGF1

      ITGA5

      MMP2

      PDGFRA

      PTGS2

      TGFB2

      WNT5A

      NTC

       * gene selection may be updated based on new research and development

      貨號GK041
      產地美國
      縮寫GQH-SWH
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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