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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        人體皮膚傷口愈合qPCR檢測套件

        英文名:Human Skin Wound Healing qPCR Array Kit

        貨號:GK041

        價格:$250

        產品說明書下載:

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        ScienCell's GeneQuery? Human Skin Wound Healing qPCR Array Kit (GQH-SWH) is designed to facilitate gene expression profiling of 88 key genes involved in the wound repair process. Wound healing is a complex process that includes many biological events, such as phagocytosis, angiogenesis, neocollagenesis, epithelialization, and collagen remodeling. It can be generally divided into 3 stages: 1) hemostasis and coagulation, 2) inflammation, and 3) proliferation and tissue remodeling. Brief examples of how included genes may be grouped according to their functions are shown below:     

        • Wound healing stages
          • Hemostasis and coagulation: IL1A, IL1B, IL6, TNF, FGF2, IGF1, TGFs, HIF1A
          • Inflammation: CXCL8, VEGFs, ELANE, CTSG, PRTN3, PLAU, TGFs, PDGFs, TNF, IFNG
          • Proliferation and tissue remodeling:  EGF, FGF7, IGF1, IFNG, CSK, RAC1, RHOA, CDC42
        • Extracellular Matrix components: COL1A2, COL3A1, COL4A1, COL14A1
        • Cellular adhesion: CDH1, ITGAs, ITGBs, MMPs, F3, FGA, TIMP1, ACTA2, ACTC1, TAGLN
        • Cytokines and chemokines: CCL2, CXCL1, CXCL2, CXCL8, IL1A, IL1B, IL4, IL6
        • Growth factors: CSF2, CSF3, EGF, CTGF, FGFs, PDGFs, TGFs

        GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

        GeneQuery? Human Skin Wound Healing qPCR Array Plate Layout*

        (8 controls in Bold and Italic)

         

         

        1

        2

        3

        4

        5

        6

        7

        8

        9

        10

        11

        12

        A

        ACTA2

        COL1A2

        CSK

        CXCL8

        FGF2

        IL10

        ITGB1

        MMP7

        PDGFRB

        RAC1

        TGFBR3

        β-actin

        B

        ACTC1

        COL3A1

        CTGF

        EGF

        FGF7

        IL1A

        ITGB3

        MMP9

        PLAT

        RHOA

        TIMP1

        GAPDH

        C

        ANGPT1

        COL4A1

        CTNNB1

        EGFR

        FIGF

        IL1B

        ITGB5

        NGF

        PLAU

        SERPINE1

        TNF

        LDHA

        D

        CCL2

        COL4A3

        CTSG

        ELANE

        HBEGF

        IL4

        ITGB6

        PDGFA

        PLAUR

        STAT3

        VEGFA

        NONO

        E

        CD40LG

        COL5A1

        CTSK

        F13A1

        HGF

        IL6

        MAPK1

        PDGFB

        PLG

        TAGLN

        VEGFB

        PPIH

        F

        CDC42

        COL5A3

        CTSV

        F3

        HIF1A

        IL6ST

        MAPK3

        PDGFC

        PRTN3

        TGFA

        VEGFC

        GDC

        G

        CDH1

        CSF2

        CXCL1

        FGA

        IFNG

        ITGA1

        MMP1

        PDGFD

        PTEN

        TGFB1

        WISP1

        PPC

        H

        COL14A1

        CSF3

        CXCL2

        FGF10

        IGF1

        ITGA5

        MMP2

        PDGFRA

        PTGS2

        TGFB2

        WNT5A

        NTC

         * gene selection may be updated based on new research and development

        貨號GK041
        產地美國
        縮寫GQH-SWH
        規格One 96-well plate, one 20ul reaction per well
        用途科研
        儲存4°C
        運輸膠冰
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        99久久香蕉国产线看观看
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