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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人成纖維細胞生長因子信號通路qPCR檢測試劑盒

      英文名:Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit

      貨號:GK040

      價格:$250

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      ScienCell's GeneQuery? Human Fibroblast Growth Factor Signaling Pathway qPCR Array Kit (GQH-FGF) is designed to facilitate gene expression profiling of 88 key genes involved in Fibroblast Growth Factor (FGF) signaling pathways. FGFs are involved in a wide variety of biological functions such as embryonic development, organogenesis, angiogenesis, tissue regeneration and wound healing. Through their binding with FGF receptors (FGFRs), FGFs activate and regulate many downstream signaling pathways, such as MAPK, JNK and STAT/NF-κB. Brief examples of how included genes may be grouped according to their functions are shown below:     

      • Secreted FGFs
        • FGF1 superfamily: FGF1, FGF2
        • FGF4 superfamily: FGF4, FGF5
        • FGF7 superfamily: FGF3, FGF7, FGF10
        • FGF8 superfamily: FGF17, FGF18
        • FGF9 superfamily: FGF9, FGF16, FGF20
      • Endocrine FGFs: FGF19, FGF23
      • Intracellular FGFs: FGF13, FGF14
      • FGF receptors: FGFR1, FGFR2, FGFR3, FGFR5
      • FGF signaling inhibitors: IL17RD, SPRY2, SPRY4, PTPN1, DUSP1, DUSP6
      • Activated downstream pathways
        • MAPK pathway: FRS2, GAB1, SOS1, PTPN11, SHC1, RAS, BRAF, MAPK1, MAPK3, MAP2K1, MAP2K2
        • PI3K/AKT pathway: AKT1, AKT2, AKT3, PI3Ks, FOXO1, FOXO3, BAD, BAX, CASP9
        • JNK pathway: MAPK8, MAPK9, MAPK14, RAC1, ATF2, MAP2K4, MAP2K6
        • STAT/NF-κB pathway: STAT1, STAT3, NFKB1, NFKB2, REL, MMP9

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human Fibroblast Growth Factor Signaling Pathway qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      AKT1

      DAG1

      FGF16

      FGF4

      FOS

      IL17RD

      MAPK14

      NFATC2

      PIK3CD

      PLCG1

      SH2B1

      β-actin

      B

      AKT2

      DUSP1

      FGF17

      FGF5

      FOXO1

      JAK2

      MAPK3

      NFATC3

      PIK3CG

      PTPN1

      SHC1

      GAPDH

      C

      AKT3

      DUSP6

      FGF18

      FGF7

      FOXO3

      KRAS

      MAPK8

      NFATC4

      PIK3R1

      PTPN11

      SOS1

      LDHA

      D

      ATF2

      ELK1

      FGF19

      FGF9

      FOXO4

      MAP2K1

      MAPK9

      NFKB1

      PIK3R2

      RAC1

      SPRY2

      NONO

      E

      BAD

      FGF1

      FGF2

      FGFR1

      FRS2

      MAP2K2

      MARCKS

      NFKB2

      PIK3R3

      RAF1

      SPRY4

      PPIH

      F

      BAX

      FGF10

      FGF20

      FGFR2

      GAB1

      MAP2K4

      MMP9

      NRAS

      PIK3R4

      REL

      SRC

      GDC

      G

      BRAF

      FGF13

      FGF23

      FGFR3

      GRB2

      MAP2K6

      NFAT5

      PIK3CA

      PIK3R5

      RELA

      STAT1

      PPC

      H

      CASP9

      FGF14

      FGF3

      FGFR5

      HRAS

      MAPK1

      NFATC1

      PIK3CB

      PIK3R6

      RELB

      STAT3

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK040
      產地美國
      縮寫GQH-FGF
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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