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      人類先天免疫反應qPCR檢測試劑盒

      英文名:Human Innate Immune Response qPCR Array Kit

      貨號:GK039

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Innate Immune Response qPCR Array Kit (GQH-IIR) facilitates gene expression profiling of key genes in the human innate immune system.  The innate immune response is activated upon microbial infection or foreign substance presentation and includes activation of the complement system and adaptive immune systems.  A separate array is available for analyzing the adaptive immune response.  Brief examples of how included genes may be grouped according to function are shown below:

      • Bacteria response: TLR1, TLR5, NOD1, LY96, TRAM1
      • Virus response: TLR3, DDX58, IFIH1, MYD88, IRAK4
      • Self recognition: TLR2, TLR4, TLR7, TLR8, CLEC4E
      • Inflammasome component: CASP1, CASP5, PYCARD, NLRP1
      • Complement system component: C3, MBL2, MASP1, CFB, CD46

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Innate Immune Response qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ATF3

      CASP5

      CLEC6A

      IFIH1

      IRAK1

      LYZ

      NAP1L1

      OTUD5

      TICAM1

      TLR5

      TRAF6

      β-actin

      B

      C3

      CD46

      CLEC7A

      IFNA1

      IRAK2

      MAP3K7

      NFAM1

      PYCARD

      TICAM2

      TLR6

      TRAM1

      GAPDH

      C

      C5

      CFB

      DDX58

      IFNB1

      IRAK3

      MAPK8

      NFATC1

      RIPK2

      TIRAP

      TLR7

      TRAM2

      LDHA

      D

      C6

      CFD

      DHX58

      IFNG

      IRAK4

      MASP1

      NFKB1

      RNF125

      TLR1

      TLR8

      TRIM25

      NONO

      E

      C7

      CFH

      EYA4

      IL18

      IRF3

      MASP2

      NFKBIE

      SYK

      TLR10

      TLR9

      TYROBP

      PPIH

      F

      C8A

      CFP

      FADD

      IL1A

      IRF7

      MAVS

      NLRP1

      TAB2

      TLR2

      TNF

      UBE2N

      GDC

      G

      CARD9

      CHUK

      HCST

      IL1B

      JUN

      MBL2

      NOD1

      TAB3

      TLR3

      TRADD

      UBE2V1

      PPC

      H

      CASP1

      CLEC4E

      HMGB1

      IL6

      LY96

      MYD88

      NOD2

      TBK1

      TLR4

      TRAF3

      UNC93B1

      NTC

       *gene selection may be updated based on new research and development

      貨號GK039
      產地美國
      縮寫GQH-IIR
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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