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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類HIF1信號響應qPCR檢測試劑盒

      英文名:Human HIF1 Signaling Response qPCR Array Kit

      貨號:GK036

      價格:$250

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      ScienCell's GeneQuery? Human HIF1 Signaling Response qPCR Array Kit (GQH-HFT) is designed to facilitate gene expression profiling of 88 key genes involved in HIF1 downstream signaling upon hypoxia-induced activation.? HIF1 activity is a major component of the human response to hypoxia.? Brief examples of how included HIF1 downstream genes may be grouped according to regulatory function are shown below:

      • Cell cycling:?DHFR, MAP3K5, POLE2, PRC1, SKP2
      • Extracellular matrix remodeling:?COL1A1, COL5A1, LOX, P4HA1, PLOD1
      • Angiogenesis:?HMOX1, MMP2, VEGFA, ANGPTL4, PGF
      • Metabolism:?PKM, STC2, DHX35, DDX11, ERO1A

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

      GeneQuery? Human HIF1 Signaling Response qPCR Array Plate Layout* (8 controls?in Bold and Italic)

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      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ABCB1

      BHLHE40

      COL18A1

      DUSP6

      HMOX1

      LOXL2

      NEK4

      PKM

      PRC1

      RRAS

      TSFM

      β-actin

      B

      ADORA2A

      BRCA1

      COL1A1

      ELK1

      IGFBP3

      MAP3K5

      NOTCH4

      PLCG2

      PSME3

      RRM2

      TXNIP

      GAPDH

      C

      ADORA2B

      CCNE1

      COL1A2

      ERO1A

      INHBA

      MCM4

      P4HA1

      PLOD1

      PTGS1

      SKP2

      UBE2S

      LDHA

      D

      AK3

      CCNE2

      COL5A1

      EXOSC2

      INHBE

      MEF2A

      P4HA2

      PLOD2

      PTPRB

      SMAD9

      VEGFA

      NONO

      E

      ANGPTL4

      CCNF

      CXCR4

      FOS

      INSR

      MLLT10

      PAM

      POLE2

      PTPRR

      STC1

      VEGFC

      PPIH

      F

      BARD1

      CDC6

      DDX11

      GADD45B

      KDM3A

      MMP2

      PDZD2

      POLR3K

      RB1

      STC2

      VLDLR

      GDC

      G

      BCL2

      CDK1

      DHFR

      GINS2

      LEPR

      MXI1

      PELI2

      PPARD

      RFC5

      TFAM

      ZNF292

      PPC

      H

      BCL6

      CDKN1C

      DHX35

      HES1

      LOX

      NDRG1

      PGF

      PPARG

      RNH1

      TRIP13

      ZWINT

      NTC

      ?

      *gene selection may be updated based on new research and development

      貨號GK036
      產地美國
      縮寫GQH-HFT
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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