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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人體缺氧反應qPCR檢測試劑盒

      英文名:Human Hypoxia Response qPCR Array Kit

      貨號:GK035

      價格:$250

      產品說明書下載:

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      Hypoxia refers to any environment with reduced oxygen content compared to normal aerobic conditions.  The hypoxic response is induced when cells are oxygen deprived and prolonged deprivation often results in physiological compensation by the local cellular environment, such as increased angiogenesis to the hypoxic region.  ScienCell's GeneQuery? Human Hypoxia Response qPCR Array Kit (GQH-HPX) is designed to facilitate gene expression profiling of 88 key genes involved in the cellular response to low-oxygen conditions.  Brief examples of how included genes may be grouped according to response type are shown below:

      • Cellular metabolism: ENO1, HK1, PFKFB3, PGK1, SLC2A1
      • Inflammation: CXCL8, IL1A, CCL2, IL6, THBD
      • Vasorelaxation: AHSP, PTGIS
      • Cell death or proliferation: BNIP3L, NOS3, PIM1, EPO
      • Angiogenesis: KDR, PDGFB, PLAU, TEK, FLT1

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Endothelial Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      AHSP

      BNIP3L

      EGLN1

      EPO

      HIF3A

      JMJD6

      OS9

      PIK3CA

      PTGIR

      SAT2

      TEK

      β-actin

      B

      ALDOA

      CA9

      EGLN2

      F2

      HK1

      KDR

      PDGFB

      PIM1

      PTGIS

      SELP

      THBD

      GAPDH

      C

      ANXA2

      CCL2

      EGLN3

      FLT1

      HRH1

      LDHA

      PDPK1

      PLAU

      PTGS2

      SENP1

      TNF

      LDHA

      D

      APEX1

      COPS5

      EGR1

      FOXO4

      ICAM1

      LGALS3

      PECAM1

      PPP3CB

      RACK1

      SLC2A1

      TP53

      NONO

      E

      ARNT

      CREBBP

      EIF4E

      GSK3B

      IFNG

      LTC4S

      PER1

      PPP3CC

      RPS6

      SP1

      TXN

      PPIH

      F

      ARNT2

      CUL2

      ENO1

      GYS1

      IL1A

      MAP3K1

      PFKFB3

      PRKCA

      RUVBL2

      STAT3

      USP20

      GDC

      G

      ARNTL

      CXCL8

      EP300

      HIF1A

      IL4

      NFKB1

      PFKFB4

      PSMC6

      RWDD3

      TCEB1

      VCAM1

      PPC

      H

      BNIP3

      EDN1

      EPAS1

      HIF1AN

      IL6

      NOS3

      PGK1

      PTGFR

      SAT1

      TCEB2

      VHL

      NTC

       

       * gene selection may be updated based on new research and development

       

      貨號GK035
      產地美國
      縮寫GQH-HPX
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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