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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人肺纖維化qPCR檢測試劑盒

      英文名:Human Pulmonary Fibrosis qPCR Array Kit

      貨號:GK034

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Pulmonary Fibrosis qPCR Array Kit (GQH-PFS) is designed to facilitate gene expression profiling of 88 key genes involved in pulmonary fibrosis. Pulmonary fibrosis is caused by excessive extracellular matrix (ECM) deposition during wound healing and tissue remodeling. The scarring of lung leads to thickened, stiff tissues and respiratory problems over time. Brief examples of how included genes may be grouped according to their functions are shown below:     

      • Pulmonary surfactant proteins: SFTPA2, SFTPAC
      • Inflammatory chemokines: CCL2, CCL3, CCL11, CCR2, CCR3, CXCL8
      • Inflammatory cytokines: PDGFA, CSF2, IL5, IL6, IL13
      • ECM remodeling: LOX, MMP1, MMP2, MMP8, PLAT, PLAU, PLG, SERPINA1, SERPINE2, TIMP1, TIMP2, TIMP3
      • Growth factors: EGF, IGF1, HGF, FGF1, FGF2, AGT, EDN1, CTGF
      • TGFB signaling pathway: SKIL, SPP1, TGFB1, TGFB2

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human Pulmonary Fibrosis qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACTA2

      CCL3

      CXCL8

      FAM13A

      HPS3

      IL13

      MMP1

      NFE2L2

      PLAU

      SFTPD

      TGFA

      β-actin

      B

      AGT

      CCL5

      CYSLTR2

      FGF1

      HPS4

      IL13RA2

      MMP13

      NFKB1

      PLG

      SKIL

      TGFB1

      GAPDH

      C

      ATP11A

      CCR2

      DSP

      FGF2

      HPS5

      IL1B

      MMP2

      NFKB2

      PTX3

      SMAD3

      TGFB2

      LDHA

      D

      BMP7

      CCR3

      EDN1

      FGF7

      HPS6

      IL5

      MMP3

      OBFC1

      SERPINA1

      SMAD7

      TIMP1

      NONO

      E

      CALCA

      CEBPB

      EGF

      GREM1

      IFNG

      IL6

      MMP8

      PARN

      SERPINE1

      SNAI1

      TIMP2

      PPIH

      F

      CCL11

      CSF2

      ELMOD2

      HGF

      IGF1

      LOX

      MMP9

      PDGFA

      SERPINH1

      SPP1

      TIMP3

      GDC

      G

      CCL18

      CSF3

      ELN

      HMOX1

      IL10

      LPAR1

      MT2A

      PDGFB

      SFTPA2

      TERC

      TIMP4

      PPC

      H

      CCL2

      CTGF

      FAM111B

      HPS1

      IL12B

      MECP2

      MUC5B

      PLAT

      SFTPC

      TERT

      TNF

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK034
      產地美國
      縮寫GQH-PFS
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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