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      人類Wnt信號靶基因qPCR檢測試劑盒

      英文名:Human Wnt Signaling Target Genes qPCR Array Kit

      貨號:GK028

      價格:$250

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      ScienCell's GeneQuery? Human Wnt Signaling Target Genes qPCR Array Kit (GQH-WTG) is designed to facilitate gene expression profiling of 88 key genes responsive to Wnt signaling activation and genes downstream of Wnt signaling induced transcription.  This kit includes genes regulated by both canonical and noncanonical Wnt signaling pathways.  Brief examples of how included downstream genes may be grouped according to their respective functions are shown below:

      • Transcription factors: HNF1A, LEF1, TCF7L1, TCF7L2, FOSL1
      • Cell cycling/growth: CCND1, CDKN2A, BIRC5, APC, TERC
      • Motility: S100A4, S100A6, NRCAM, KRT1, FN1
      • Angiogenesis: EDN1, PLAU, VEGFA, NOTCH1, EDNRA
      • Cancer misregulation: TWIST1, RET, JUN, NEDD9, MLLT10

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Wnt Signaling Target Genes qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ABCB1

      CCND1

      DOT1L

      EP300

      HES1

      L1CAM

      MSL1

      NOTCH2

      PRL

      SOX2

      TNFRSF19

      β-actin

      B

      AMER1

      CD44

      EDN1

      FGF18

      HNF1A

      LEF1

      MYC

      NRCAM

      PTGS2

      SOX9

      TWIST1

      GAPDH

      C

      APC

      CDH1

      EDN3

      FGF20

      ID2

      MET

      MYCN

      PEX2

      RET

      SP5

      TWIST2

      LDHA

      D

      ATOH1

      CDKN2A

      EDNRA

      FGF4

      IGF1

      MLLT10

      NANOG

      PITX2

      RUNX2

      TBC1D9

      VCAN

      NONO

      E

      BIRC5

      CDX1

      EGFR

      FN1

      IL6

      MMP14

      NANOS2

      PLAU

      S100A4

      TCF7L1

      VEGFA

      PPIH

      F

      BMP4

      CLDN1

      EGR1

      FOSL1

      IRS1

      MMP2

      NDP

      PLAUR

      S100A6

      TCF7L2

      WISP1

      GDC

      G

      CACNA1G

      CTLA4

      EGR2

      GBX2

      JUN

      MMP7

      NEDD9

      POSTN

      SOST

      TERC

      WISP2

      PPC

      H

      CCNA2

      CXCL8

      EMP1

      GJA1

      KRT1

      MMP9

      NOTCH1

      PPARD

      SOX17

      TIAM1

      YAP1

      NTC

       * gene selection may be updated based on new research and development

      貨號GK028
      產地美國
      縮寫GQH-WTG
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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