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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人類非經典Wnt信號通路qPCR檢測試劑盒

      英文名:Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

      貨號:GK027

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Noncanonical Wnt Signaling Pathways qPCR Array Kit (GQH-WAP) is designed to facilitate gene expression profiling of 88 key genes involved in alternative Wnt pathways.  This kit emphasizes genes involved in planar cell polarity signaling and calcium signal transduction.  Brief examples of how included genes may be grouped according to function are shown below:

      • Planar cell polarity: DAAM1, CELSR1, FAT1, FZD7, VANGL1
      • Calcium: CASR, CALM1, FOSL1, FZD4, NFATC1
      • Signaling modulator: LRP5, LRP6, SPRY1, EFNB, CASR
      • Receptors: WNT2B, WNT4, WNT5A, WNT9A, WNT11
      • Target genes: RHOA, RACGAP1, CREBBP, CFL1, FUZ

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Noncanonical Wnt Signaling Pathways qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ATN1

      CAPN2

      CREBBP

      DGKE

      FAT4

      FZD6

      ITPR3

      PFN1

      RACGAP1

      SPRY1

      WNT4

      β-actin

      B

      AXIN1

      CASR

      CREBZF

      DVL1

      FLNA

      FZD7

      LIMK1

      PLCD1

      RGS18

      TLR4

      WNT5A

      GAPDH

      C

      AXIN2

      CDX2

      CSNK1E

      DVL2

      FOSL1

      GSK3B

      LRP5

      PLCE1

      RGS3

      VANGL1

      WNT5B

      LDHA

      D

      CALM1

      CELSR1

      DAAM1

      DVL3

      FUZ

      HGS

      LRP6

      PLCG1

      RHOA

      VANGL2

      WNT6

      NONO

      E

      CAMK2A

      CELSR2

      DCHS1

      EFNB1

      FZD2

      INTU

      MAPK8

      PRICKLE1

      ROR1

      WNT11

      WNT7A

      PPIH

      F

      CAMK2B

      CELSR3

      DCHS2

      FAT1

      FZD3

      INVS

      NFATC1

      PRICKLE2

      ROR2

      WNT16

      WNT7B

      GDC

      G

      CAMK4

      CFL1

      DGKB

      FAT2

      FZD4

      ITPR1

      NFATC2

      PRKCD

      RYK

      WNT2B

      WNT9A

      PPC

      H

      CAPN1

      CFL2

      DGKD

      FAT3

      FZD5

      ITPR2

      PCDH8

      PTK7

      SIAH2

      WNT3A

      WNT9B

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK027
      產地美國
      縮寫GQH-WAP
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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      99久久香蕉国产线看观看

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