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      人類Wnt信號通路qPCR檢測試劑盒

      英文名:Human Wnt Signaling Pathway qPCR Array Kit

      貨號:GK026

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Wnt Signaling Pathway qPCR Array Kit (GQH-WNT) is designed to facilitate gene expression profiling of 88 key genes involved in Wnt signaling transduction.  The Wnt signaling pathway is well known for its role in carcinogenesis and embryonic development.  This kit focuses on canonical Wnt signaling.  Brief examples of how included genes may be grouped according to purpose are shown below:

      • Ligands: all known WNTs
      • Receptors: FZD1-10, SMO, LRP5/6, RSPO1-4, LGR4-6
      • Receptor transducers:  DVL1, DVL2, DVL3, CSNK1E, MARK1
      • Transcription factors: CTNNB1, TCF7L1, TCF7L2, LEF1, PYGO1
      • Regulatory elements: NLK, NKD1, AES, SFRP1, LRP1

       

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Wnt Signaling Pathway qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      AES

      CSNK2A1

      DKKL1

      FZD2

      GSK3A

      LRP1

      PORCN

      RSPO4

      SMO

      WNT16

      WNT6

      β-actin

      B

      APC

      CTNNB1

      DVL1

      FZD3

      GSK3B

      LRP5

      PPP2R4

      RYK

      TCF7L1

      WNT2

      WNT7A

      GAPDH

      C

      AXIN1

      CTNNBIP1

      DVL2

      FZD4

      HNF1A

      LRP6

      PYGO1

      SFRP1

      TCF7L2

      WNT2B

      WNT7B

      LDHA

      D

      AXIN2

      CUL1

      DVL3

      FZD5

      KREMEN1

      MARK1

      ROR1

      SFRP2

      TLE1

      WNT3

      WNT8A

      NONO

      E

      BCL9

      DKK1

      FBXW7

      FZD6

      LEF1

      MARK2

      ROR2

      SFRP4

      WNT1

      WNT3A

      WNT8B

      PPIH

      F

      CDC37

      DKK2

      FRZB

      FZD7

      LGR4

      MARK3

      RSPO1

      SKP1

      WNT10A

      WNT4

      WNT9A

      GDC

      G

      CSNK1A1L

      DKK3

      FZD1

      FZD8

      LGR5

      NKD1

      RSPO2

      SKP2

      WNT10B

      WNT5A

      WNT9B

      PPC

      H

      CSNK1E

      DKK4

      FZD10

      FZD9

      LGR6

      NLK

      RSPO3

      SMARCA4

      WNT11

      WNT5B

      ZNRF3

      NTC

       

       * gene selection may be updated based on new research and development

      貨號GK026
      產地美國
      縮寫GQH-WNT
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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