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      人類Notch信號通路qPCR檢測試劑盒

      英文名:uman Notch Signaling Pathway qPCR Array Kit

      貨號:GK025

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Notch Signaling Pathway qPCR array kit (GQH-NCH) is designed to facilitate gene expression profiling of 88 key genes involved in the human Notch molecular pathway with an emphasis on Notch1 signal transduction.  Brief examples of how included genes may be grouped according to their roles are shown below:

       

      • Canonical ligands: JAG1, JAG2, DLL1, DLL3DLL4
      • Noncanonical ligands: WNT1, DNER, CNTN1, NOV, SHH
      • Receptors: NOTCH1, NOTCH2, NOTCH3, NOTCH4
      • Transcriptional repressors: HEY1, HEY2, ESR1, HES1, HES7
      • Proteolysis: ADAM17, PSEN1, FURIN, POFUT1, PSENEN

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis. 

      GeneQuery? Human Notch Signaling Pathway qPCR Array Plate Layout* (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACBD3

      CDKN1A

      DLL1

      DVL2

      GSK3B

      IL2RA

      MAML3

      NCOR2

      NRARP

      PSENEN

      SMAD2

      β-actin

      B

      ADAM10

      CIR1

      DLL3

      EGFL7

      HES1

      JAG1

      MEF2C

      NEURL1

      NUMB

      PTF1A

      SMO

      GAPDH

      C

      ADAM17

      CLTA

      DLL4

      ESR1

      HES5

      JAG2

      MFAP2

      NFKB2

      NUMBL

      RBPJ

      SNW1

      LDHA

      D

      APH1A

      CNTN1

      DNAAF3

      ESR2

      HES7

      JUN

      MFAP5

      NOTCH1

      PCSK5

      RFNG

      SPEN

      NONO

      E

      AXIN1

      CNTN6

      DNER

      FBXW7

      HEY1

      LFNG

      MFNG

      NOTCH2

      POFUT1

      RITA1

      TBP

      PPIH

      F

      CCND1

      CTNNB1

      DNM1

      FHL1

      HEY2

      MAGI2

      MIB1

      NOTCH3

      POGLUT1

      RUNX1

      TCF7L2

      GDC

      G

      CDK2

      DLK1

      DTX1

      FURIN

      HEYL

      MAML1

      MIB2

      NOTCH4

      PSEN1

      SHH

      THBS2

      PPC

      H

      CDK8

      DLK2

      DTX4

      GATA3

      HIF1A

      MAML2

      MLLT4

      NOV

      PSEN2

      SIRT1

      WNT1

      NTC

       

       

      * gene selection may be updated based on new research and development

      貨號GK025
      產地美國
      縮寫GQH-NCH
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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