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      我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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      人內皮細胞分化qPCR檢測試劑盒

      英文名:Human Endothelial Cell Differentiation qPCR Array Kit

      貨號:GK023

      價格:$250

      產品說明書下載:

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      ScienCell's GeneQuery? Human Endothelial Cell Differentiation qPCR Array Kit (GQH-ECD) is designed to facilitate gene expression profiling of 88 key genes to help characterize endothelial cells during lineage differentiation and is based on extensive literature research.  Brief examples of how included genes may be grouped according to specification markers are shown below:

      • Circulating early endothelial progenitors: MAFB, RB1, ADORA3, MYCL1, NCOA2
        • Circulating late endothelial progenitors: FOXF1, ITGAM, SIX4, EGFR, CREB5
        • Hemangioblasts: GATA1, LMO2, PROM1, RUNX1, TAL1
        • Lymphatic endothelial cells: FLT4, LYVE1, PDPN, PROX1, FGFR3
        • EndMT: ACTA2, SMAD3, S100A4, TWIST, BMP7

      Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

       

       GeneQuery? Human Endothelial Cell Differentiation qPCR Array Plate Layout *

      (8 controls in Bold and Italic)

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      ACE

      CD34

      EGFR

      FOXF1

      IL1R1

      LYVE1

      NFYA

      PODXL

      RUNX1

      SLC2A9

      TAL1

      ACTB

      B

      ACTA2

      CD36

      EPAS1

      GATA1

      ITGAM

      MAFB

      NODAL

      PROM1

      S100A4

      SMAD1

      TBX18

      GAPDH

      C

      ADORA3

      CDH1

      EFNB2

      GATA6

      JAG1

      MAP3K10

      NOTCH1

      PROX1

      SEMA6D

      SMAD3

      TEK

      LDHA

      D

      BMP10

      CDH5

      EPHB4

      GDF2

      JDP2

      MAPK14

      NR2F2

      PTGFR

      SHH

      SMAD5

      TGFBR3

      NONO

      E

      BMP4

      CFC1

      ETV1

      GSC

      KDR

      MCAM

      NRP1

      RB1

      SIGLEC1

      SNAI1

      TRPS1

      PPIH

      F

      BMP7

      CREB5

      FGFR3

      GSK3B

      LILRB5

      MYCL

      NRP2

      RHOA

      SIX4

      SOX18

      TWIST1

      GDC

      G

      CD163

      CTNNB1

      FLT1

      HDAC5

      LMO2

      NCOA2

      PDPN

      ROCK1

      SLAMF8

      T

      VCAM1

      PPC

      H

      CD248

      CXADR

      FLT4

      ICAM2

      LPAR1

      NFATC1

      PECAM1

      ROR2

      SLC16A3

      TAGLN

      ZEB1

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK023
      產地美國
      縮寫GQH-ECD
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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