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      人類T細胞受體信號通路qPCR檢測試劑盒

      英文名:Human T Cell Receptor Signaling Pathway qPCR Array Kit

      貨號:GK021

      價格:$250

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      ScienCell's GeneQuery? Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:     

      • T cell receptor complex components: CD3D, CD3E, CD3G
      • Co-receptors: CD4, CD8A, CD8B
      • Co-inhibitors: PDCD1, CTLA4
      • Co-stimulators: ICOS, CD28
      • Kinases: FYN, ZAP70
      • MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
      • PI3K/AKT pathway: PI3Ks, AKT1, AKT2
      • IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG

      GeneQuery? qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

      GeneQuery? Human T Cell Receptor Signaling Pathway qPCR Array Plate Layout*

      (8 controls in Bold and Italic)

       

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      AKT1

      CD274

      CD8A

      DYRK1A

      HRAS

      MALT1

      NCK1

      NFKBIE

      PDPK1

      PPP3CB

      RELA

      β-actin

      B

      AKT2

      CD28

      CD8B

      DYRK2

      ICOS

      MAP2K1

      NCK2

      NRAS

      PIK3CA

      PPP3R1

      SOS1

      GAPDH

      C

      BCL10

      CD3D

      CDC42

      FOS

      IKBKB

      MAP2K7

      NFATC1

      NRON

      PIK3CB

      PRKCA

      SOS2

      LDHA

      D

      CALM1

      CD3E

      CDK4

      FYN

      IKBKG

      MAP3K7

      NFATC2

      PAK1

      PIK3R1

      PRKCQ

      TEC

      NONO

      E

      CARD11

      CD3G

      CHUK

      GRAP2

      ITK

      MAP3K8

      NFATC3

      PAK2

      PIK3R2

      PTCRA

      TNF

      PPIH

      F

      CBL

      CD4

      CTLA4

      GRB2

      JUN

      MAPK1

      NFKB1

      PAK3

      PIK3R3

      PTPN6

      VAV1

      GDC

      G

      CBLB

      CD80

      DAG1

      GSK3A

      KRAS

      MAPK3

      NFKBIA

      PAK4

      PLCG1

      RAF1

      VAV2

      PPC

      H

      CBLC

      CD86

      DLG1

      GSK3B

      LCP2

      MAPK9

      NFKBIB

      PDCD1

      PPP3CA

      RASGRP1

      ZAP70

      NTC

       

      * gene selection may be updated based on new research and development

      貨號GK021
      產地美國
      縮寫GQH-TRS
      規格One 96-well plate, one 20ul reaction per well
      用途科研
      儲存4°C
      運輸膠冰
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