99久久香蕉国产线看观看

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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        鐵檢測試劑盒

        英文名:Iron Assay

        貨號:8448

        價格:$266

        產品說明書下載:

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        Iron is an element that plays an essential role in a number of biological processes. Iron is bound to several proteins such as the oxygen-transporting hemoglobin in the red blood cells and oxygen storage protein myoglobin in the muscle. It is also stored by ferritin and hemosiderin. Inorganic iron contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase. In this colorimetric assay, ferric carrier protein will dissociate ferric into solution under acidic conditions. After they are reduced to the ferrous ions, these ions react with chromogen to produce a stable colored complex and give absorbance at 590 nm. The color intensity thus formed is directly proportional to the concentration of iron in the sample. A specific chelate chemical is included in the buffer to block copper ion interference.
        貨號8448
        產地美國
        縮寫IR
        規格 100 tests
        用途科研
        儲存-20度
        運輸干冰
        No Publication available at this time

        Question:

        Hello, I wanted some clarification for part B: preparation of test samples for the Iron Assay Kit (Catalog #8448). I am confused what it means by " 4 volumes of the assay buffer", and "93 volumes of assay buffer (8448a), 2 volumes reductant (8448e) and 5 volumes sample reagents (8448d)." Does volumes just mean any unit of volume as long as that ratio is used? Thank you, Lauren Nardacci

        Answers:

        • Posted by?Manoj Sharma?on Wednesday, January 6, 2016

          Hi Lauren,

          We have revised the preparation as follows:

          B. Preparation of test samples
          1. One volume of cells or tissues can be homogenized in 4 volumes of the assay buffer (8448a). Centrifuge the?samples at 13,000 ×g for 10 minutes to remove insoluble material.
          2. Prepare a fresh working buffer by mixing assay buffer (8448a), reductant (8448e) and sample reagents?(8448d) at 93:2:5 ratio (volume ratio). (e.g., 930 μL mixing assay buffer, 20 μL reductant, and 50 μL sample?reagents)
          3. Mix sample (cell lysate or serum) with equal volume of working buffer.
          4. Centrifuge the mixed sample 13,000 ×g for 5 minutes and take the supernatant as the test samples for the?iron assay.
          5. Test samples should be serial diluted to make sure the readings are within the standard curve range. Prepare?test samples to a final volume of 50 μL/well on the 96-well flat bottom plate.

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        99久久香蕉国产线看观看
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