99久久香蕉国产线看观看

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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        人星形膠質細胞 HA

        英文名:Human Astrocytes

        貨號:1800

        價格:$686

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        ? ? ? 在哺乳動物的大腦中,絕大部分細胞是星形膠質細胞。它們形態各異,并與神經元各個部分緊密相連,因此可能具有多種功能。星形膠質細胞為神經元提供結構,營養和代謝上的支持并調節突觸活動。此外,星形膠質細胞可提示許多神經疾病的病理過程。腦卒中及其他神經損傷后,正常星形膠質細胞功能受損,可嚴重影響神經元的存活。腦損傷后的長期恢復,通過軸突生長,突觸可塑性,或神經元再生,也影響星形膠質細胞表面分子的表達和營養因子釋放。大量的研究已經表明,星形膠質細胞是在中樞神經系統中的最功能多樣化的細胞群。在體外研究中,星形膠質細胞的培養為探索細胞多樣性提供了重要工具。


        ? ? ? ScienCell實驗室的HA提取自大腦皮層,原代凍存,冰凍運輸。每管細胞密度超過1×106/ml。細胞經GFAP免疫熒光鑒定。本細胞經檢測不含HSCHIV-1,HBV,HCV,支原體,細菌,酵母菌和真菌。如采用ScienCell實驗室特制的培養基,可保證此細胞10次倍增。

        推薦培養基:星形膠質細胞培養基(AM, Cat. #1801)

        貨號1800
        產地美國
        縮寫HA
        規格5 x 10^5 /1ml
        用途科研
        儲存液氮
        運輸干冰
        1.) Zhou Y, Hayashi I, Wong J, Tugusheva K, Renger JJ, Zerbinatti C. (2014) 'Intracellular Clusterin Interacts with Brain Isoforms of the Bridging Integrator 1 and with the Microtubule-Associated Protein Tau in Alzheimer's Disease.'

        2.) Monticone M, Taherian R, Stigliani S, Carra E, Monteghirfo S, Longo L, Daga A, Dono M, Giaretti W, Castagnola P. (2014) 'NAC, Tiron and Trolox Impair Survival of Cell Cultures Containing Glioblastoma Tumorigenic Initiating Cells by Inhibition of Cell Cycle Progression.'

        3.) Cruz-Orengo L, Daniels BP, Dorsey D, Basak SA, Grajales-Reyes JG, McCandless EE, Piccio L, Schmidt RE, Cross AH, Crosby SD, Klein RS. (2014) 'Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility.' J clin invest.

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        6.) Zhou Y, Hayashi I, Wong J, Tugusheva K, Renger JJ, Zerbinatti C. (2014) "Intracellular Clusterin Interacts with Brain Isoforms of the Bridging Integrator 1 and with the Microtubule-Associated Protein Tau in Alzheimer's Disease." PloS one. 9: e103187.

        7.) Urich E, Patsch C, Aigner S, Graf M, Iacone R, Freskgrd P. (2013) "Multicellular self-assembled spheroidal model of the blood brain barrier." Sci Rep. 3: 1500.

        8.) Abdelmoaty S, Wigerblad G, Bas DB, Codeluppi S, Fernandez-Zafra T, El-Awady ES, Moustafa Y, Abdelhamid AE, Brodin E, Svensson CI. (2013) "Spinal Actions of Lipoxin A4 and 17(R)-Resolvin D1 Attenuate Inflammation-Induced Mechanical Hypersensitivity and Spinal TNF Release." PLoS One. 8: e75543.

        9.) Cucullo L, Hossain M, Tierney W, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system: Cerebrovascular physiology in a box." BMC Neuroscience. 14:18

        10.) Vijayaraghavan S, Karami A, Aeinehband S, Behbahani H, Grandien A, Nilsson B, Ekdahl KN, Lindblom RP, Piehl F, Darreh-Shori T. (2013) "Regulated Extracellular Choline Acetyltransferase Activity - The Plausible Missing Link of the Distant Action of Acetylcholine in the Cholinergic Anti-Inflammatory Pathway." PLoS One. 8: e65936

        11.) Li J, Bonifati S, Hristov G, Marttila T, Valmary Degano S, Stanzel S, Schnolzer M, Mougin C, Aprahamian M, Grekova SP, Raykov Z, Rommelaere J, Marchini A. (2013) 'Synergistic combination of valproic acid and oncolytic parvovirus H?1PV as a potential therapy against cervical and pancreatic carcinomas.'

        12.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) 'Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration.'

        13.) Arscott W, Tandle AT, Zhao S, Shabason JE, Gordon IK, Schlaff CD, Zhang G, Tofilon PJ, Camphausen KA. (2013) "Ionizing Radiation and Glioblastoma Exosomes: Implications in Tumor Biology and Cell Migration." Transloncol. 6: 638-48.

        14.) Li J, Bonifati S, Hristov G, Marttila T, Valmary?Degano S, Stanzel S, Schnolzer M, Mougin C, Aprahamian M, Grekova SP, Raykov Z, Rommelaere J, Marchini A. (2013) "Synergistic combination of valproic acid and oncolytic parvovirus H?1PV as a potential therapy against cervical and pancreatic carcinomas." EMBO Mol Med. 5: 1537-55.

        15.) Corti S, Nizzardo M, Simone C, Falcone M, Donadoni C, Salani S, Rizzo F, Nardini M, Riboldi G, Magri F, Zanetta C, Faravelli I,Bresolin N, Comi GP. (2012) "Direct reprogramming of human astrocytes into neural stem cells and neurons." Exp Cell Res. 318: 1528-41.

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        ?

        1、Question:

        Which genes could represent good housekeeping genes for RT-qPCR analysis of these cells? Any suggestions?

        Answers:

        We recommend β-actin, GAPDH, LDHA, NONO, and PPIH to serve as housekeeping genes for astrocyte qRT-PCR. We also recommend using multiple housekeeping genes for data normalization. In addition, in a qRT-PCR assay, we recommend using Genomic DNA (gDNA) Control (GDC) to detect possible gDNA contamination, and Positive PCR Control (PPC) to test whether samples contain inhibitors or other factors that may negatively affect gene expression results. ScienCell offers GeneQuery? Human Astrocyte Cell Biology qPCR Array Kit for astrocyte qRT-PCR analysis.

        2、Question:

        WAre this cell lines?

        Answers:

        They are primary cells, not immortalized cell lines. thanks for your interest

        3、Question:

        What is the recommended maximum number of passages that the primary human astrocytes can be grown to without losing phenotype? Is there a recommended freezing protocol?

        Answers:

        Human Astrocytes can be subcultured for 4-5 passages. However, for primary cells, the markers such as GFAP may be lost during each passage, even though they are still astrocytes. Therefore, we recommend that customers use the cells at early passage without expanding or passaging too much. We do not recommend re-freezing the cells for future use. thanks for your interest

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        99久久香蕉国产线看观看

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