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        我的購物車>> 中國區首家總代 專業十六載 打造細胞生物學精品
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        您當前所在位置:首 頁 > 產品展示 > 人多能干細胞 > 分化和多能干細胞來源的細胞 人多能干細胞來源的神經干細胞 HiPSC-NSC

        人多能干細胞來源的神經干細胞 HiPSC-NSC

        英文名:HiPSC-derived Neural Stem Cells

        貨號:1650

        價格:$655

        產品說明書下載:

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        Human Neural Stem Cells (Cat. #1650) from ScienCell Research Laboratories are differentiated from a human induced pluripotent stem cell line (hiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HDF-hiPSC are efficiently converted to neural epithelium using HPSC Neural Induction Medium (PSCNIM, Cat. #5931), a serum-free medium for rapid and efficient neural induction of human pluripotent stem cells (hPSCs). Synergistic inhibition of glycogen synthase kinase 3 (GSK3) and transforming growth factor b (TGF-b) differentiates HDF-hiPSCs to homogenous neural stem cells (NSC) in the presence of human leukemia inhibitory factor (LIF) within 7 days. The derived NSC are characterized by immunofluorescence with antibodies specific to nestin and SOX2. The cell population is highly pure: >90% of cells express Nestin and >80% of cells are SOX2 positive. After reviving, NSC can be maintained in HPSC Neural Induction Medium as an adherent culture or form neural spheres in EGF and bFGF containing neural stem cell medium. The cells are able to form rosette and neural tube-like structures after being passaged and plated at high density on Matrigel coated culture vessels in neural stem medium containing EGF and bFGF. HiPSC-NSC can be further expanded for months as a monolayer on MatrigelTM coated culture vessels or in suspension as neural spheres when seeded on ultra-low binding culture vessels in neural stem cell medium. NSC are multipotent and able to differentiate into various neuronal and glial subtypes. Specific patterning cues, such as SHH, retinoic acid and FGF8, can be added after reviving to direct the cells to different neural lineages. HiPSC-NSC are cryopreserved at passage 0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
        貨號1650
        產地美國
        縮寫HiPSC-NSC
        規格1 x 10^6 /1ml
        用途科研
        儲存液氮
        運輸干冰
        No Publication available at this time

        Question:

        Do you know if they would survive plating on poly-D-Lysine coated plates for either overnight or shorter culturing ? we have an end point application which requires plating in the absence of Matrigel coating. We would plan to propagate the NSC cell stock on matrigel and only plate on poly-D-lys for the acute assay. Thanks

        Answers:

        • Posted by Manoj Sharma on Thursday, January 12, 2017

          Dear Marlene,

          We recommend plating the cells on Matrigel to help retain the multi-potential of the cells. It is possible that plating the cells on poly-D-Lysine could cause the cells to spontaneously differentiate. Do you want the cells to remain neural stem cells or do you want them to differentiate? If you do choose to plate the cells on poly-D-lysine, I would try to keep the density pretty high to help keep the cells in the neural stem cell state (but of course I cannot guarantee how the cells will respond).

          Please let me know if you have any further questions.

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        99久久香蕉国产线看观看
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