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Human Neural Stem Cells (Cat. #1650) from ScienCell Research Laboratories are differentiated from a human induced pluripotent stem cell line (hiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HDF-hiPSC are efficiently converted to neural epithelium using HPSC Neural Induction Medium (PSCNIM, Cat. #5931), a serum-free medium for rapid and efficient neural induction of human pluripotent stem cells (hPSCs). Synergistic inhibition of glycogen synthase kinase 3 (GSK3) and transforming growth factor b (TGF-b) differentiates HDF-hiPSCs to homogenous neural stem cells (NSC) in the presence of human leukemia inhibitory factor (LIF) within 7 days. The derived NSC are characterized by immunofluorescence with antibodies specific to nestin and SOX2. The cell population is highly pure: >90% of cells express Nestin and >80% of cells are SOX2 positive. After reviving, NSC can be maintained in HPSC Neural Induction Medium as an adherent culture or form neural spheres in EGF and bFGF containing neural stem cell medium. The cells are able to form rosette and neural tube-like structures after being passaged and plated at high density on Matrigel coated culture vessels in neural stem medium containing EGF and bFGF. HiPSC-NSC can be further expanded for months as a monolayer on MatrigelTM coated culture vessels or in suspension as neural spheres when seeded on ultra-low binding culture vessels in neural stem cell medium. NSC are multipotent and able to differentiate into various neuronal and glial subtypes. Specific patterning cues, such as SHH, retinoic acid and FGF8, can be added after reviving to direct the cells to different neural lineages. HiPSC-NSC are cryopreserved at passage 0 and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

貨號	1650
產地	美國
縮寫	HiPSC-NSC
規格	1 x 10^6 /1ml
用途	科研
儲存	液氮
運輸	干冰

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Question:

Do you know if they would survive plating on poly-D-Lysine coated plates for either overnight or shorter culturing ? we have an end point application which requires plating in the absence of Matrigel coating. We would plan to propagate the NSC cell stock on matrigel and only plate on poly-D-lys for the acute assay. Thanks

Answers:

• Posted by Manoj Sharma on Thursday, January 12, 2017

  Dear Marlene,

  We recommend plating the cells on Matrigel to help retain the multi-potential of the cells. It is possible that plating the cells on poly-D-Lysine could cause the cells to spontaneously differentiate. Do you want the cells to remain neural stem cells or do you want them to differentiate? If you do choose to plate the cells on poly-D-lysine, I would try to keep the density pretty high to help keep the cells in the neural stem cell state (but of course I cannot guarantee how the cells will respond).

  Please let me know if you have any further questions.