貨號 | 1650 |
產地 | 美國 |
縮寫 | HiPSC-NSC |
規格 | 1 x 10^6 /1ml |
用途 | 科研 |
儲存 | 液氮 |
運輸 | 干冰 |
Do you know if they would survive plating on poly-D-Lysine coated plates for either overnight or shorter culturing ? we have an end point application which requires plating in the absence of Matrigel coating. We would plan to propagate the NSC cell stock on matrigel and only plate on poly-D-lys for the acute assay. Thanks
Dear Marlene,
We recommend plating the cells on Matrigel to help retain the multi-potential of the cells. It is possible that plating the cells on poly-D-Lysine could cause the cells to spontaneously differentiate. Do you want the cells to remain neural stem cells or do you want them to differentiate? If you do choose to plate the cells on poly-D-lysine, I would try to keep the density pretty high to help keep the cells in the neural stem cell state (but of course I cannot guarantee how the cells will respond).
Please let me know if you have any further questions.