腎臟近曲小管上皮細胞在腎臟功能中起了關鍵作用。它們重吸收腎小球濾液中幾乎所有的葡萄糖和氨基酸,允許其它無營養價值的物質排放至尿液中。在先天性疾病、新陳代謝疾病以及炎癥反應中,該細胞出現于損傷部位附近,可分泌如細胞因子和趨化因子等炎癥介質,并可通過產生IL-8影響和直接參與白細胞趨化反應,進而參與急性炎癥反應的過程。在炎癥、腎移植術后及新月體腎小球腎炎中,該細胞可表達IL-2R α型和第二類MHC分子。腎近曲小管上皮細胞的培養為研究管狀細胞與腎臟疾病之間的關聯提供了一個有用的體外模型。
Sciencell研究實驗室的人腎近曲小管上皮細胞是從人的腎中分離得到的,一代凍存,冰凍運輸。每管細胞密度超過5×10^5/ml。該細胞可通過細胞因子-18、-19和波形蛋白特異性抗體的免疫熒光進行鑒定。本細胞經檢測不含HIV-1、HBV、HCV、支原體、細菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養基,可保證此細胞15倍增殖。
推薦培養基:上皮細胞培養基?(EpiCM, Cat. #4101)?
貨號 | 4100 |
產地 | 美國 |
縮寫 | HRPTEpiC |
規格 | 5 x 10^5/1ml |
用途 | 科研 |
儲存 | 液氮 |
運輸 | 干冰 |
1.) Van der Hauwaert C, Savary G, Gnemmi V, Glowacki F, Pottier N, Bouillez A, Maboudou P, Zini L, Leroy X, Cauffiez C, Perrais M, Aubert S. (2013) "Isolation?and?characterization?of a?primary?proximal?tubular?epithelial cell?model?from?human kidney?by?CD10/CD13?double?labeling."?PLoS One.?8: e66750.
2.) Du T, Zou X, Cheng J, Wu S, Zhong L, Ju G, Zhu J, Liu G, Zhu Y, Xia S. (2013) "Human Wharton?s jelly-derived mesenchymal stromal cells reduce renal fibrosis through induction of native and foreign hepatocyte growth factor synthesis in injured tubular epithelial cells."?Stem Cell Res Ther. 4: 59.
3.) Dudas PL, Sague SL, Elloso MM, Farrell FX. (2011) "Proinflammatory/Profibrotic Effects of Interleukin-17A on Human Proximal Tubule Epithelium."?Nephron Exp Nephrol. 117: e114-23.
4.) Zhang H, Tasnim F, Ying JY, Zink D. (2009) "The impact of extracellular matrix coatings on the performance of human renal cells applied in bioartificial kidneys."Biomaterials. 30: 2899-911.
5.) Hatsukari I, Singh P, Hitosugi N, Messmer D, Valderrama E, Teichberg S, Chaung W, Gross E, Schmidtmayerova H, Singhal PC. (2007) "DEC-205-mediated internalization of HIV-1 results in the establishment of silent infection in renal tubular cells."?J Am Soc Nephrol. 18: 780-7.
?
?